Antioxidant and antitumor activities of Artemisia campestris and Thymelaea hirsuta from southern Tunisia
Antioxidant and antitumor activities of Artemisia campestris and Thymelaea hirsuta from southern Tunisia
复制标题
DOI：
10.1016/j.fct.2010.11.003
复制
发表时间：
2011-02-01
影响因子：
4.3
通讯作者：
Campra Madrid, Pablo
中科院分区：
文献类型：
--
作者：
Akrout, Ahmed;Alarcon Gonzalez, Lidia;Campra Madrid, Pablo
用四甲基偶氮唑盐(四甲基偶氮唑盐)比色法(DPPH法、ABTS法和β-胡萝卜素法)对突尼斯南部采集的野油菜精油和野油菜和毛细辛的乙醇-水、正己烷和水提取物的抗氧化活性和对人结肠癌HT-29细胞生长的抑制作用进行了研究。除正己烷提取物外，所有油菜提取物在高浓度(100mU g/ml)下对人结肠癌HT-29细胞生长的抑制作用在19.5%到64.4%之间。对毛刺参而言，除浸提液外，其余提取物(正己烷和乙醇-水)均具有抗肿瘤活性(分别为对照生长的58.2%和65.5%)，野油菜乙醇-水和浸提液的抗氧化活性、多酚和类黄酮类化合物含量均高于毛刺参。结果表明，野油菜的抗肿瘤活性与抗氧化活性呈正相关，且与多酚和类黄酮类物质含量呈正相关。野油菜精油等提取物对HT-29细胞具有显著的抗肿瘤活性，值得进一步研究其对结肠癌的化学预防和治疗作用。(C)2010爱思唯尔有限公司。保留所有权利。
The essential oil of Artemisia campestris and the ethanol-water, hexane and water extracts of A. campestris and Thymelaea hirsuta collected in southern of Tunisia were investigated for their antioxidant (DPPH, ABTS and beta-carotene methods) and antitumor growth inhibition of human colon cancer HT-29 cells using MTT test activities.All the A. campestris extracts tested at high concentrations (100 mu g/ml) showed activity ranging from 19.5% for essential oil to 64.4% of negative control growth for infusion extract, except the hexane extract. With T. hirsuta, all the extracts tested (hexane and ethanol-water), except the infusion extract, also exhibited antitumor activity (58.2% and 65.5% of control growth respectively).The ethanol-water and infusion extracts of A. campestris showed higher antioxidant activity, polyphenol and flavonoid contents than those of T. hirsuta. These results show that there is a positive correlation between the antitumor activity and the antioxidant activity, and of these two activities and with the levels of polyphenols and flavonoids.The essential oil and the other extracts of A. campestris, which exhibited significant antitumor activity against the HT-29 cells deserve further research into the chemoprevention and treatment of colon cancer. (C) 2010 Elsevier Ltd. All rights reserved.