Disruption of the E1 and E2 reading frames of HPV 16 in cervical carcinoma is associated with poor prognosis
Disruption of the E1 and E2 reading frames of HPV 16 in cervical carcinoma is associated with poor prognosis
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DOI：
10.1097/00004347-199804000-00009
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发表时间：
1998-04-01
影响因子：
2.4
通讯作者：
Johansson, B
中科院分区：
文献类型：
--
作者：
Kalantari, M;Karlsen, F;Johansson, B
应用聚合酶链反应（PCR）技术，对158例HPV 16型阳性宫颈癌标本的E1和E2阅读框进行了定位。使用跨越整个基因的引物扩增阅读框。在分析的样品中，23%显示E1引物没有扩增，29%显示E2引物没有扩增。有一个重叠，但不完全的身份，E1和E2破坏组之间。使用跨越E1和E2阅读框子部分的引物进一步分析所有E1和E2阴性样本，这些引物共同覆盖了整个基因。35例E1阴性标本中，11例E1基因3'端特异性扩增阳性。E2基因的几个不同的亚基可以从大多数样品（37/46）的整个基因阴性扩增。发现了5类模式，其中E2基因的所有亚基或5 '端、中间或3'端的亚基被破坏。尽管在宫颈癌中发现HPV 16基因组E1-E2区域存在不同的破坏/缺失模式，但5'端破坏是E1和E2中最常见的。E1/E2破坏的癌患者的生存率低于无E1/E2破坏的患者，E1破坏是最重要的预后因素。
The E1 and E2 reading frames of 158 cervical carcinoma samples containing human papillomavirus (HPV) 16 were mapped using polymerase chain reaction (PCR). The reading frames were amplified using primers spanning the entire genes. Of the analyzed samples, 23% showed no amplification with the E1 primers and 29% showed no amplification with the E2 primers. There was an overlap, but not complete identity, between the E1- and E2-disrupted groups. All E1- and E2-negative samples were further analyzed with primers spanning subsections of the E1 and E2 reading frames, which together covered the entire genes. Of the 35 samples negative for E1, 11 were positive in specific amplification of the 3' end of the E1 gene. Several different subsections of E2 could be amplified from most samples negative for the entire gene (37/46). Five classes of patterns were found, in which either all subsections of the E2 gene or subsections in the 5', middle, or 3' end were disrupted. Although a variable pattern of disruption/deletion In the E1-E2 area of the HPV 16 genome was found in cervical carcinoma, the 5' end disruption was the most common one in both E1 and E2. Patients with carcinomas showing disruptions in E1/E2 had a poorer survival than those without such changes, and E1 disruptions were the most important prognostically.