Efficient plant regeneration system from leaf discs of zonal (Pelargonium x hortorum) and two scented (P-capitatum and P-graveolens) geraniums
Efficient plant regeneration system from leaf discs of zonal (Pelargonium x hortorum) and two scented (P-capitatum and P-graveolens) geraniums
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DOI：
10.1007/s11240-005-6308-y
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发表时间：
2005-11-01
影响因子：
3
通讯作者：
Dorion, N
中科院分区：
文献类型：
--
作者：
Hassanein, A;Dorion, N
以带状和芳香天竺葵的叶盘为外植体，建立了一套高效、快速的植株再生体系。研究了不同外植体、培养基和培养条件对不定芽诱导的影响。无论基因型如何，从4 - 5周龄的离体生长的植物中取出的叶盘比从温室生长的植物中取出的叶盘更有效地用于芽再生。黑暗是芽再生的刺激因素，其中NAA与两种细胞分裂素的组合效果最好。在半浓度MS +0.5 mg l（-1）NAA +1 mg l（-1）BAP和玉米素的培养基上，在黑暗条件下（每个外植体11.4个芽），可从鸭茅叶圆片获得100%的直接芽再生。在相同的培养基和培养条件下，所有的香紫苏叶圆片也表现出直接芽再生（7.3个芽/外植体）。对于P. x hortorum，100%的叶盘在MS培养基上进行芽再生，该培养基补充有0.2 mg l（-1）NAA和0.5 mg l（-1）BAP和玉米素，在黑暗中（每个外植体8.8个芽）或在弱光条件下，0.2 mg l（-1）NAA和1 mg l（-1）BAP和玉米素（每个外植体7.5个芽）。对于该物种，芽伸长的最佳结果是在用Phytagel 0.3%（v/v）凝胶化的半强度MS培养基上获得的。无论基因型，所有的芽生根容易转移到稀释的MS培养基（MS/2）含有1毫克升（-1）IAA。适应植物生长正常，并在温室条件下开花。对驯化植株的叶片进行流式细胞仪分析表明，所有的香天竺葵植株都与母株相似，而表现出强烈生长的P. xhortorum植株中有71%是四倍体。
An efficient and rapid plant regeneration system was established for zonal and scented geraniums using leaf discs as explants. Several explants, medium and culture conditions were studied to optimize shoot induction. Leaf discs taken from 4 - 5 weeks old in vitro grown plants, whatever the genotype, were more effective for shoot regeneration than those taken from greenhouse grown plants. Darkness proved to be a stimulating factor for shoot regeneration and the combination between NAA and two cytokinins gave the best results. Direct shoot regeneration (100%) was obtained from leaf discs of P. capitatum on half-strength MS medium supplemented with 0.5 mg l(-1) NAA in combination with 1 mg l(-1) of BAP and zeatin in darkness (11.4 shoots per explant). In the same medium and culture conditions, all P. graveolens leaf discs also exhibited direct shoot regeneration (7.3 shoots per explant). For P. x hortorum, 100% of leaf discs underwent shoot regeneration on a MS medium supplemented with 0.2 mg l(-1) NAA in combination with 0.5 mg l(-1) of BAP and zeatin in darkness (8.8 shoots per explant) or under low light conditions with 0.2 mg l(-1) NAA and 1 mg l(-1) of BAP and zeatin (7.5 shoots per explant). For this species, the best results for shoot elongation were obtained on half-strength MS medium gelled with Phytagel 0.3% (v/v). Whatever the genotype, all shoots rooted readily when transferred to diluted MS medium (MS/2) containing 1 mg l(-1) IAA. Acclimatized plants grew normally and flowered in greenhouse conditions. Flow cytometry analysis made on leaves of acclimatized plants revealed that all the scented geranium plants are similar to mother plants while 71% of P. x hortorum plants which showed strong growth were tetraploid.