Development and Field Evaluation of a System to Collect Mosquito Excreta for the Detection of Arboviruses
Development and Field Evaluation of a System to Collect Mosquito Excreta for the Detection of Arboviruses
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用于虫媒病毒检测的收集蚊子排泄物系统的开发和现场评估
DOI：
10.1093/jme/tjz031
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发表时间：
2019
影响因子：
2.1
通讯作者：
S. Ritchie
中科院分区：
文献类型：
--
作者：
Dagmar B. Meyer;A. L. Ramírez;A. F. Hurk;N. Kurucz;S. Ritchie
摘要蚊媒疾病是全球关注的主要公共卫生问题，病原体的早期检测是实施媒介管理和控制战略的关键。现有的病原体检测方法包括筛选哨兵动物的抗体和分析蚊子的病原体存在。虽然这些方法是有效的，但它们也昂贵、劳动强度大，并且在后勤方面具有挑战性。为了解决这些局限性，开发了一种新的方法，即在涂有蜂蜜的核酸保存卡上收集蚊子唾液，然后通过分子分析来检测病原体。然而，蚊子在吸食这些卡片时只会排出少量的唾液，可能会导致假阴性。蚊子排出的另一种体液比唾液更多的是排泄物，最近的实验室实验表明，在蚊子排泄物中可以检测到一系列由蚊子传播的病原体。在目前的研究中，我们改进了光诱蚊器和被动式诱蚊器来收集它们的排泄物，并在野外评估中评估了它们的效果。从这些现场采集中，我们检测到了西尼罗河、罗斯河和墨累谷脑炎病毒。我们的发现表明，捕蚊器很容易被改装成收集排泄物，而且这个系统有可能加强对病原体的检测。
Abstract Mosquito-borne diseases are a major public health concern globally and early detection of pathogens is critical to implement vector management and control strategies. Existing methods for pathogen detection include screening sentinel animals for antibodies and analyzing mosquitoes for pathogen presence. While these methods are effective, they are also expensive, labor-intense, and logistically challenging. To address these limitations, a new method was developed whereby mosquito saliva is collected on honey-coated nucleic acid preservation cards which are analyzed by molecular assays for detection of pathogens. However, mosquitoes only expel small amounts of saliva when feeding on these cards, potentially leading to false negatives. Another bodily fluid that is expelled by mosquitoes in larger volumes than saliva is excreta, and recent laboratory experiments have demonstrated that a range of mosquito-borne pathogens can be detected in mosquito excreta. In the current study, we have modified light and passive mosquito traps to collect their excreta and assessed their efficacy in field evaluations. From these field-collections, we detected West Nile, Ross River, and Murray Valley encephalitis viruses. Our findings suggest that mosquito traps are easily modified to collect excreta and, that this system has the potential to enhance detection of pathogens.