Antigen-free medium for cultivation of Haemophilus influenzae, AFH-medium.
Antigen-free medium for cultivation of Haemophilus influenzae, AFH-medium.
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用于培养流感嗜血杆菌的无抗原培养基，AFH-培养基。
DOI：
10.1111/j.1699-0463.1972.tb00151.x
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
P. Branefors
中科院分区：
文献类型：
--
作者：
P. Branefors
描述了一种无抗原培养基，AFH培养基，用于培养流感嗜血杆菌。培养基由胰酶酵母自溶物制备，通过Sephadex G-25柱过滤。对于中碱，只使用含有低分子物质的馏分。试验了添加不同量的盐和缓冲液，并研究了培养基初始pH值的影响。葡萄糖氧化酶试验测定生长过程中消耗的葡萄糖量。AFH培养基使所有被试菌株生长旺盛。在液体培养基中的细菌产量为1 × 1010-1 × 1011 / ml，在固体培养基上过夜培养的菌落直径为1 - 2 mm。
An antigen-free medium, AFH medium, for cultivation of H. influenzae is described. The medium is prepared from trypticase-yeast autolysate, filtrated through a Sephadex G-25 column. For the medium base only the fractions containing low molecular material were utilized. Addition of different amounts of salt and buffer was tested and the effect of the initial pH of the medium was studied. The amount of glucose consumed during growth was measured with a glucose oxidase test. The AFH medium provided luxuriant growth of all tested strains. The bacterial yield in the fluid medium was 1 times 1010-1 times 1011 per ml and overnight cultures on the solid medium resulted in colonies 1–2 mm in diameter.