The structure of GABPα/β:: An ETS domain ankyrin repeat heterodimer bound to DNA
The structure of GABPα/β:: An ETS domain ankyrin repeat heterodimer bound to DNA
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DOI：
10.1126/science.279.5353.1037
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发表时间：
1998-02-13
期刊：
影响因子：
56.9
通讯作者：
Wolberger, C
中科院分区：
文献类型：
--
作者：
Batchelor, AH;Piper, DE;Wolberger, C
GA结合蛋白(GABP)是由两个结构不同的亚基组成的转录调节蛋白。阿尔法亚基包含一个DNA结合域，它是ETS家族的成员之一，而贝塔亚基包含一系列锚蛋白重复序列。在2.15埃分辨率下测定了一个含有GABPα/β结构域-锚蛋白重复序列与DNA结合的三元络合物的晶体结构。该结构显示了Ets结构域蛋白如何利用Ets结构域和羧基末端延伸招募伙伴蛋白，并提供了由一组锚定蛋白重复序列形成的广泛的蛋白质-蛋白质界面的视图。该结构还揭示了GABPαETS结构域如何与其核心GGA DNA识别基序结合。
GA-binding protein (GABP) is a transcriptional regulator composed of two structurally dissimilar subunits. The alpha subunit contains a DNA-binding domain that is a member of the ETS family, whereas the beta subunit contains a series of ankyrin repeats. The crystal structure of a ternary complex containing a GABP alpha/beta ETS domain-ankyrin repeat heterodimer bound to DNA was determined at 2.15 angstrom resolution. The structure shows how an ETS domain protein can recruit a partner protein using both the ETS domain and a carboxyl-terminal extension and provides a view of an,extensive protein-protein interface formed by a set of ankyrin repeats. The structure also reveals how the GABP alpha ETS domain binds to its core GGA DNA-recognition motif.