Arabidopsis transcriptional activators CBF1, CBF2, and CBF3 have matching functional activities
Arabidopsis transcriptional activators CBF1, CBF2, and CBF3 have matching functional activities
复制标题
DOI：
10.1023/b:plan.0000040902.06881.d4
复制
发表时间：
2004-03-01
影响因子：
5.1
通讯作者：
Thomashow, MF
中科院分区：
文献类型：
--
作者：
Gilmour, SJ;Fowler, SG;Thomashow, MF
当拟南芥暴露于低温时，编码称为CBF1，CBF2和CBF3（也分别称为DREB1b，DREB1c和DREB1a）的转录因子的小基因家族被快速诱导，随后表达CBF靶向基因，CBF调节子，其作用是提高抗冻性。CBF1、2和3蛋白质虽然在氨基酸序列上高度相似，但并不相同，这就提出了这些蛋白质是否具有相同功能的问题。本文通过比较CBF基因过表达对拟南芥生长发育、脯氨酸和糖组成、抗冻性以及基因表达的影响来探讨这一问题。综上所述，结果支持CBF1、2和3转录激活因子具有冗余功能活性的结论。
When Arabidopsis is exposed to low temperature a small gene family encoding transcription factors known as CBF1, CBF2, and CBF3 (also referred to as DREB1b, DREB1c, and DREB1a, respectively) is rapidly induced followed by expression of CBF-targeted genes, the CBF regulon, which act to bring about an increase in freezing tolerance. The CBF1, 2 and 3 proteins, though highly similar in amino acid sequence, are not identical, raising the question of whether the proteins have the same functions. Here we explored this issue by comparing the effects that overexpression of each CBF gene had on Arabidopsis growth and development, proline and sugar composition, freezing tolerance and gene expression. Taken together, the results support the conclusion that the CBF1, 2 and 3 transcriptional activators have redundant functional activities.