Unequal modification of the thermal survival responses of clonal human colon carcinoma cell lines by decreased pH.
Unequal modification of the thermal survival responses of clonal human colon carcinoma cell lines by decreased pH.
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通过降低 pH 值对克隆人结肠癌细胞系的热存活反应进行不平等的改变。
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
J. Leith
中科院分区：
文献类型：
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作者：
J. Leith
我们已经调查了两个密切相关的人类结肠肿瘤细胞系（克隆A和克隆D）的反应，以高温细胞在体外杀死在两个条件下的环境酸度（pH值7.40和6.75）。在pH7.40，在42.5摄氏度的长期暴露下，两条线表现出双相响应曲线，耐热性出现在约4小时的连续加热。发现克隆D人肿瘤系比克隆A系更耐高温灭活。在pH 6.75时，两种细胞系均表现出对42.5 ℃下加热杀灭的敏感性增加，但发现两种人肿瘤细胞系表现出的效果不相同（即pH降低并不只是作为剂量调节剂）。具体地说，克隆A在存活的耐热区域（即加热时间为4小时或更长）中表现出比克隆D大得多的热敏感性。临床上，这一结果表明，虽然潜在的pH值敏感的异质性肿瘤高温细胞杀伤可能会有所不同，在一个给定的肿瘤内的肿瘤细胞亚群之间的随机方式，增加热敏感性发现可能会产生相同的加热时间为不同的亚群的isosurvival结果。
We have investigated the responses of two closely related human colon tumour lines (clone A and clone D) to hyperthermic cell killing in vitro under two conditions of environmental acidity (pH values 7.40 and 6.75). At pH 7.40, under prolonged exposure to 42.5 degrees C, both lines exhibited biphasic response curves with thermotolerance appearing at about 4 h of continuous heating. It was found that the clone D human tumour line was more resistant to hyperthermic inactivation than the clone A line. At pH 6.75, both lines exhibited increased sensitivity to heating killing at 42.5 degrees C, but it was found that the effect was unequally demonstrated by the two human tumour lines (i.e. decreased pH was not acting simply as a dose modifying agent). Specifically, clone A exhibited a much greater hyperthermic sensitivity in the thermotolerant region of survival (i.e. a heating time of 4 h or more) than did clone D. Clinically, this result would suggest that although potential pH sensitization of heterogeneous tumours to hyperthermic cell killing may vary in a random manner among tumour cell subpopulations within a given tumour, the increased heat sensitivity found may produce isosurvival results for equal heating times for the different subpopulations.
两种异质性人类癌症亚群在体外对 X 射线照射的差异反应。
影响因子：
11.2
作者：
Leith,JT;Dexter,DL;DeWyngaert,JK;Zeman,EM;Chu,MY;Calabresi,P;Glicksman,AS
通讯作者：
Glicksman,AS
pH 对 42-45 度单次或分次热处理的影响。
影响因子：
11.2
作者：
Gerweck,LE;Dahlberg,WK;Greco,B
通讯作者：
Greco,B
pH 值对细胞对单剂量和分剂量热疗反应的影响。
影响因子：
11.2
作者：
Gerweck,LE;Jennings,M;Richards,B
通讯作者：
Richards,B