Effect of oxidants on small intestinal brush border membranes and colonic apical membranes - a comparative study
Effect of oxidants on small intestinal brush border membranes and colonic apical membranes - a comparative study
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DOI：
10.1016/s1532-0456(02)00250-8
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发表时间：
2003-03-01
影响因子：
3.9
通讯作者：
Balasubramanian, KA
中科院分区：
文献类型：
--
作者：
Prabhu, R;Balasubramanian, KA
本研究比较了大鼠小肠刷状缘膜（BBM）和结肠根尖膜（CAM）的组成及其对各种氧化剂的体外暴露敏感性。BBM和CAM在脂质组成、糖含量、碱性磷酸酶（ALP）活性和胆固醇/磷脂比方面存在差异。将BBM和CAM暴露于黄嘌呤+黄嘌呤氧化酶（X-XO）产生的超氧化物或过氧化叔丁基（tBuOOH）和过氧化氢（H2O2）等过氧化物中，分析其ALP活性、过氧化参数和膜脂质的变化。BBM和CAM暴露于超氧化物导致ALP活性降低，过氧化参数如蛋白质羰基含量、丙二醛和共轭二烯增加。超氧化物暴露也导致脂质改变，特别是某些磷脂。这些改变是由超氧化物歧化酶或别嘌呤醇阻止的。过氧化物没有明显的影响。这些结果表明，BBM和CAM都容易受到超氧化物的影响，超氧化物可导致膜脂过氧化和降解，特别是某些磷脂。(C) 2002爱思唯尔科学有限公司版权所有。
This study compares composition of the rat small intestinal brush border membranes (BBM) and colonic apical membranes (CAM) and their susceptibility to in vitro exposure to various oxidants. Differences were observed between BBM and CAM in their lipid composition, sugar content, alkaline phosphatase (ALP) activity and cholesterol/ phospholipid ratio. BBM and CAM were exposed to superoxide generated by xanthine+xanthine oxidase (X-XO) or peroxides such as tertiary butyl hydroperoxide (tBuOOH) and hydrogen peroxide (H2O2) and alterations in ALP activity, peroxidation parameters and membrane lipids were analyzed. Exposure of BBM and CAM to superoxide resulted in decrease in ALP activity and increase in peroxidation parameters such as protein carbonyl content, malondialdehyde and conjugated diene. Superoxide exposure also resulted in lipid alterations specifically in certain phospholipids. These alterations were prevented either by superoxide dismutase or by allopurinol. Peroxides did not have any significant effect. These results suggest that both BBM and CAM are susceptible to superoxide, which can bring about peroxidation and degradation of membrane lipids specifically, certain phospholipids. (C) 2002 Elsevier Science Inc. All rights reserved.