A unified method for the assay of uridine diphosphoglucuronyltransferase activities toward various aglycones using uridine diphospho[U-14C]glucuronic acid.
A unified method for the assay of uridine diphosphoglucuronyltransferase activities toward various aglycones using uridine diphospho[U-14C]glucuronic acid.
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使用尿苷二磷酸[U-14C]葡萄糖醛酸测定尿苷二磷酸葡萄糖醛酸基转移酶对各种苷元活性的统一方法。
DOI：
10.1016/0003-2697(80)90655-7
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发表时间：
1980
影响因子：
2.9
通讯作者：
Gessner,T
中科院分区：
文献类型：
--
作者：
Bansal,SK;Gessner,T
描述了一种使用UDP-[U14-C]葡萄糖醛酸测定各种糖苷配基的UDP-葡萄糖醛酸转移酶活性的方法。通过薄层色谱法将反应期间形成的放射性葡糖苷酸结合物与未代谢的UDP-[U-14 C]葡糖醛酸和其他未表征的放射性产物分离。通过比较其薄层色谱上的Rf值与几种真正的葡萄糖醛酸苷的Rf值，以及通过在不存在或存在β-葡萄糖醛酸苷酶抑制剂d-糖二酸-1，4-内酯的情况下用β-葡萄糖醛酸苷酶水解来鉴定缀合物。该方法适用于测定各种糖苷配基的酶活性。它已被用于11个糖苷配基，并能够扩展到许多其他。
A method is described for the assay of UDP-glucuronyltransferase activity for various aglycones using UDP-[U14-C]glucuronic acid. The radioactive glucuronide conjugates formed during the reactions were separated from unmetabolized UDP-[U-14C]glucuronic acid, and from other uncharacterized radioactive products, by thin-layer chromatography. The conjugates were identified by comparison of their Rfvalues on thin-layer chromatography with those of several authentic glucuronides, and by hydrolysis with β-glucuronidase in the absence or presence of d-saccharic acid-1,4-lactone, an inhibitor of β-glucuronidase. The procedure is general for the determination of the enzymic activity with various aglycones. It has been utilized for eleven aglycones and is capable of extension to many others.