MOLECULAR-CLONING OF CDNA-ENCODING A BOVINE SELENOPROTEIN P-LIKE PROTEIN CONTAINING 12 SELENOCYSTEINES AND A (HIS-PRO) RICH DOMAIN INSERTION, AND ITS REGIONAL EXPRESSION
MOLECULAR-CLONING OF CDNA-ENCODING A BOVINE SELENOPROTEIN P-LIKE PROTEIN CONTAINING 12 SELENOCYSTEINES AND A (HIS-PRO) RICH DOMAIN INSERTION, AND ITS REGIONAL EXPRESSION
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DOI：
10.1016/0169-328x(94)00007-2
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发表时间：
1995-06-01
期刊：
影响因子：
--
通讯作者：
SUMINO, K
中科院分区：
文献类型：
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作者：
SAIJOH, K;SAITO, N;SUMINO, K
当含有丰富的蛋白质在牛小脑皮质的cDNA进行克隆，克隆似乎编码硒蛋白P样蛋白被分离。其cDNA插入片段的编码核苷酸序列与大鼠和人硒蛋白P cDNA具有高度同源性，但含有12个而不是10个TGA（在推导的氨基酸中有12个而不是10个硒代半胱氨酸），一个CACTCC（His-Ser）和七个CATCCC（His-Pro）的串联重复，3'端非翻译区比大鼠肝硒蛋白P短约890个碱基。RT-PCR显示无重复序列的mRNA的双链。串联重复序列及其邻近区域由CAC/TCC/AC/T相似的基序组成。因此，这些蛋白质包括一个（His-Pro）丰富的结构域，具有轻微的负自由能变化，无论是否有串联重复。据报道，这种His-Pro重复存在于果蝇的节段基因配对或同源盒蛋白Om（1D）中。此外，这两个硒蛋白P-样蛋白mRNA和硒蛋白P mRNA的表达在大脑的所有领域，但最突出的是在小脑皮质，海马和嗅球。这些结果表明，这些硒蛋白的可能性是主要的硒载体在大脑中发挥作用的神经或胶质细胞的形态反应。
When cDNA containing proteins enriched in the bovine cerebellar cortex were cloned, a clone which seemed to encode a selenoprotein P-like protein was isolated. The coding nucleotide sequence of its cDNA insert displayed high homology to rat and human selenoprotein P cDNA but contained 12 rather than 10 TGAs (12 rather than 10 selenocysteines in deduced amino acids), a tandem repeat of one CACTCC (His-Ser) and seven CATCCCs (His-Pro), and a 3' untranslated region approximately 890 bases shorter than that of rat liver selenoprotein P. RT-PCR using a set of primers flanking to the repeat displayed the existense of mRNA without the repeat. The tandem repeat and its adjacent region consisted of a similar motif of CAC/TCC/AC/T. Thus, these proteins included a (His-Pro) rich domain with a slightly negative free energy change irrespective of having the tandem repeat or not. Such His-Pro repeats reportedly exist in the segmentation gene paired or homeobox protein Om(1D) of Drosophila. Moreover, both this selenoprotein P-like protein mRNA and selenoprotein P mRNA were expressed in all the areas of the brain but most prominently in the cerebellar cortex, hippocampus, and olfactory bulb. These findings suggest the possibility that these selenoproteins are major selenium carriers in the brain and play a role in the morphological response of nerve or glial cells.