Molecular cloning and characterization of Hearm caspase-1 from Helicoverpa armigera
Molecular cloning and characterization of Hearm caspase-1 from Helicoverpa armigera
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DOI：
10.1007/s11033-007-9100-8
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发表时间：
2008-09
影响因子：
2.8
通讯作者：
Dan-Tong Yang;Lian‐Qin Chai;Jinxing Wang;Xiaofan Zhao
中科院分区：
文献类型：
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作者：
Dan-Tong Yang;Lian‐Qin Chai;Jinxing Wang;Xiaofan Zhao
Caspase家族的成员在程序性细胞死亡(PCD)中发挥着核心和进化的作用，PCD在发育过程中移除不需要的、受损的和危险的细胞，以维持体内平衡。本论文描述了棉铃虫caspase基因的克隆和功能鉴定，命名为Hearm caspase-1。该基因全长1,350个碱基，包含一个885个碱基的开放阅读框架(ORF)，编码294个氨基酸组成的心脏caspase-1酶原。该蛋白与果蝇Sf caspase-1和果蝇黑腹果蝇的Caspase-1高度同源，并且具有高度保守的五肽QACQG，这是已知的caspase的催化位点，表明它是棉铃虫的效应caspase。Northern杂交和RT-PCR分析表明，Hearm caspase-1在胚胎以及摄食和游荡幼虫的脂肪体、中肠和血细胞中都有表达。心脏caspase-1在血细胞中的表达似乎与蜕皮激素的脉搏有关，并被蜕皮激素激动剂RH-2485上调，这意味着心脏caspase-1的激活受蜕皮激素的调节。
Members of the caspase family play a central and evolutionary role in programmed cell death (PCD), which removes unwanted, damaged and dangerous cells during development to maintain homeostasis. In this paper, we describe the cloning and characterization of a caspase from the cotton bollworm,Helicoverpa armigera, named Hearm caspase-1. The 1,350 bp full-length cDNA contains an 885 bp open reading frame (ORF) that encodes a Hearm caspase-1 proenzyme of 294 amino acids. The deduced protein is highly homologous toSpodoptera frugiperdaSf caspase-1 andDrosophila melanogasterICE and has the highly conserved pentapeptide QACQG, the recognized catalytic site of caspases, suggesting that it is an effector caspase of the cotton bollworm. Northern blot and RT-PCR analyses demonstrate that Hearm caspase-1 is expressed in embryos and the fat body, midgut and haemocytes of feeding and wandering larvae. Expression of Hearm caspase-1 in the haemocytes appears to be correlated with the pulse of ecdysone, and it is up-regulated by ecdysone agonist RH-2485, implying that Hearm caspase-1 activation is regulated by ecdysone.