Periodicity as a Factor in the Generation of Isotropic Compressive Growth Stress Between Microfibrils in Cell Wall Formation during a Twenty-Four Hour Period
Periodicity as a Factor in the Generation of Isotropic Compressive Growth Stress Between Microfibrils in Cell Wall Formation during a Twenty-Four Hour Period
复制标题
周期性是二十四小时内细胞壁形成过程中微原纤维之间产生各向同性压缩生长应力的一个因素
DOI：
10.1515/hf.2000.079
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
T. Okuyama
中科院分区：
文献类型：
--
作者：
Masato Yoshida;Y. Hosoo;T. Okuyama
摘要利用场发射扫描电镜和X射线能谱微区分析研究了柳杉分化管胞次生壁形成过程中细胞壁物质沿径向壁最内表面沿着沉积的生长应力的产生。当树木的膨压低（16：00 h）和当压力高（6：00 h）时采集样品。在16：00 h采集的样品中，纤维素微纤丝沉积在径向壁的最内表面上是明显的，并且很少发现无定形材料;相反，在6：00 h采集的样品中，纤维素微纤丝很少观察到，并且无定形材料是普遍的。然而，在用硅藻土去除无定形材料后，纤维素微纤丝是明显的。辐射壁内表面钾浓度6：00 h大于16：00 h。在纤维素处理后，在6：00 h收集的样品中测量的钾浓度降低到与在16：00 h收集的样品中的钾浓度相当的水平，因此发现钾与纤维素微纤丝上的无定形材料相关联。无定形物质可能是半纤维素和单纤维素的基质，在发育中的细胞壁的内表面上丰富的物质，特别是在分化管胞的膨胀期间（由于高膨压）。沉积在纤维素微纤丝之间的膨胀间隙中的半纤维素和木质素基质和间隙的日常膨胀可能产生各向同性的压缩应力（生长应力）。本文证明了细胞壁形成的周期性，作为一个结果，在分化的细胞壁的压缩生长周期。
Summary Field emission scanning electron microscopy and energy-dispersive X-ray microanalysis were used to investigate the generation of growth stress in connection with the deposition of cell wall materials along the innermost surface of radial walls during secondary wall formation of differentiating tracheids of Cryptomeria japonica. Samples were collected when the turgor pressure of the tree was low (16:00 h), and when the pressure was high (6:00 h). In samples collected at 16:00 h, cellulose microfibrils deposited on the innermost surface of radial walls were clearly evident and amorphous material was rarely found. Conversely, in samples collected at 6:00 h, cellulose microfibrils were rarely observed and the amorphous material was prevalent. Cellulose microfibrils were evident, however, after removing the amorphous material with chlorite. The concentration of potassium on the inner surface of radial walls was greater at 6:00 h than at 16:00 h. After chlorite treatment, the concentration of potassium measured in the samples collected at 6:00 h decreased to a level equivalent to that in samples collected at 16:00 h and thus potassium was found to be associated with the amorphous material on the cellulose microfibrils. The amorphous material is probably a matrix of hemicellulose and monolignol, material that is abundant on the inner surface of developing cell walls, especially during expansion (as a result of high turgor pressure) of differentiating tracheids. A matrix of hemicellulose and lignin deposited in the expanded gaps between cellulose microfibrils and daily expansion of the gaps probably produces an isotropic compressive stress (growth stress). This paper demonstrates periodicity in cell wall formation as a result of the cycles of compressive growth in the differentiating cell wall.