GRF-interacting factor1 (gif1) regulates shoot architecture and meristem determinacy in maize
GRF-interacting factor1 (gif1) regulates shoot architecture and meristem determinacy in maize
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GRF 相互作用因子 1 (GIF1) 调节玉米芽结构和分生组织决定性
DOI：
10.1105/tpc.17.00791
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Zuxin Zhang
中科院分区：
文献类型：
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作者：
Dan Zhang;Wei Sun;Renee Singh;Yuanyuan Zheng;Zheng Cao;Manfei Li;China Lunde;Sarah Hake;Zuxin Zhang
植物结构是不确定和确定的细胞命运平衡的结果。具有不确定命运的细胞位于分生组织中，由产生侧生器官的多能细胞群组成。分生组织细胞也存在于茎间组织中，为节间提供细胞，并在叶缘形成叶宽。我们鉴定了一个玉米(Zea Mays)突变体，它在平衡确定性和不确定性方面存在缺陷。该突变体具有狭窄的叶片和较短的节间，表明叶片和茎中的不确定细胞减少。相反，突变体不能控制新梢分生组织的不确定性。花序分生组织束化，而确定的腋生分生组织变得不确定。定位克隆确定生长调节因子相互作用因子1(Gif1)是负责基因。gif1mRNA积累在茎分生组织的不同区域，与受突变影响的组织一致。我们确定了哪些生长调节因子与GIF1相互作用，并进行了RNA-seq分析。许多已知在花序构型中起作用的基因在gif1中有差异表达。染色质免疫沉淀鉴定出一些差异表达的基因是GIF1的直接靶点。与这些不同的直接和间接靶标的相互作用有助于解释玉米GIF1矛盾的表型。这些结果为深入了解gif1的生物学功能提供了依据。
Plant architecture results from a balance of indeterminate and determinate cell fates. Cells with indeterminate fates are located in meristems, comprising groups of pluripotent cells that produce lateral organs. Meristematic cells are also found in intercalary stem tissue, which provides cells for internodes, and at leaf margins to contribute to leaf width. We identified a maize (Zea mays) mutant that has a defect in balancing determinacy and indeterminacy. The mutant has narrow leaves and short internodes, suggesting a reduction in indeterminate cells in the leaf and stem. In contrast, the mutants fail to control indeterminacy in shoot meristems. Inflorescence meristems are fasciated, and determinate axillary meristems become indeterminate. Positional cloning identifiedgrowth regulating factor-interacting factor1(gif1) as the responsible gene.gif1mRNA accumulates in distinct domains of shoot meristems, consistent with tissues affected by the mutation. We determined which GROWTH REGULATING FACTORs interact with GIF1 and performed RNA-seq analysis. Many genes known to play roles in inflorescence architecture were differentially expressed ingif1. Chromatin immunoprecipitation identified some differentially expressed genes as direct targets of GIF1. The interactions with these diverse direct and indirect targets help explain the paradoxical phenotypes of maize GIF1. These results provide insights into the biological functions ofgif1.