Hepatic nucleases. 1. Methods for the specific determination and characterization in rat liver.
Hepatic nucleases. 1. Methods for the specific determination and characterization in rat liver.
复制标题
肝核酸酶。
DOI：
10.1111/j.1432-1033.1975.tb02292.x
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
P. Baudhuin
中科院分区：
文献类型：
--
作者：
J. Bartholeyns;C. Peeters;H. Reychler;P. Baudhuin
为了研究核酸酶的亚细胞定位，确保匀浆的方法。大鼠肝脏的核糖核酸酶活性是由于三种酶的最佳pH不同而产生的。对于酸性核糖核酸酶（最佳 pH 5.3），可以通过在 pH 5 下进行孵育来避免其他核糖核酸酶的干扰。中性核糖核酸酶（最佳 pH 7.6）的区别在于其对来自肝匀浆上清液的天然抑制剂的敏感性。比较 50°C 酸性介质中预处理前后的活性，可以对碱性核糖核酸酶进行特异性测量（最佳 pH 8.8）。还描述了测定肝匀浆中两种脱氧核糖核酸酶和作用于聚核糖腺苷酸的酶的最佳条件。比较了这些不同核酸酶的活性并研究了它们的一些特性。
With a view to the study of the subcellular localization of nucleases, methods ensuring the homogenates. The ribonuclease activity of rat liver is due to the three enzymes with different pH optimun. For acid ribonuclease (pH optimun 5.3), it is possible to avoid interference from the other ribonucleases by performing the incubation at pH 5. Neutral ribonuclease (pH optimum 7.6) is differentiated by relying on its sensitivity to the natural inhibitor from the supernatant of liver homogenate. Comparison of activities before and after pretreatment at 50 degrees C in acid medium permits the specific measurement of alkaline ribonuclease (pH optimum 8.8). The optimal conditions for the determination in liver homogenates of two deoxyribonucleases and of an enzyme acting on polyriboadenylate are also described. The activity of these various nucleases is compared and some of their properties are investigated.