Murine pregnancy decidua produces a unique immunosuppressive molecule related to transforming growth factor beta-2.
Murine pregnancy decidua produces a unique immunosuppressive molecule related to transforming growth factor beta-2.
复制标题
小鼠妊娠蜕膜产生一种与转化生长因子β-2相关的独特免疫抑制分子。
作者：
David A. Clark;K. Flanders;D. Banwatt;W. Millar;J. Manuel;J. Stedronska;B. Rowley
小鼠同种异体妊娠中的非 T 小淋巴细胞抑制细胞在体外释放有效的免疫抑制因子，该因子被兔抗转化生长因子 (TGF)-β 中和。先前的研究表明，蜕膜抑制因子（DSF）比 TGF-β 1 小，在本文中，我们表明 DSF 在 HPLC 筛分柱上的洗脱也晚于 TGF-β 2。尽管如此，DSF 具有与 TGF-β 类似的促进 NRK 成纤维细胞贴壁依赖性生长的能力。使用针对 TGF-β 1 或 β 2 的火鸡抗体，我们发现 DSF 与 TGF-β 2 而不是 TGF-β 1 相关，并且通过使用一组针对 TGF 亚型的鼠 mAb 证实了这种关系。 PAGE和Western印迹显示HPLC纯化的DSF中的TGF-β2反应分子略小于TGF-β2，约为20至23kDa。因此，DSF 分子与 TGF-β2 密切相关，但从蜕膜中释放时，其大小不同。 TGF-β 2 相关蜕膜抑制因子也从同孕 C.B.-17 严重联合免疫缺陷 (SCID) 和怀孕 SCID-BG (C57BL/6 背景) 小鼠的蜕膜中获得，证实了 DSF 产生缺乏 T 或 B 细胞依赖性，以及与小鼠成功妊娠相关的蜕膜产生 TGF-β 相关抑制因子的普遍性。
Non-T small lymphocytic suppressor cells in murine allopregnancy release a potent immunosuppressive factor in vitro that is neutralized by rabbit anti-transforming growth factor (TGF)-beta. Previous studies have suggested that the decidual suppressor factor (DSF) is smaller than TGF-beta 1, and in this paper, we show that DSF on HPLC-sieving columns also elutes later than TGF-beta 2. Nevertheless, DSF has the ability to promote anchorage-independent growth of NRK fibroblasts similar to TGF-beta s. Using turkey antibodies specific for TGF-beta 1 or beta 2, we show that DSF is related to TGF-beta 2 rather than TGF-beta 1, and this relationship was confirmed by using a panel of murine mAb to TGF-subtypes. PAGE and Western blotting showed that the TGF-beta 2-reactive molecules in HPLC-purified DSF was slightly smaller than TGF-beta 2 and approximately 20 to 23 kDa. The DSF molecule is therefore closely related to TGF-beta 2 but as released from decidua, differs in size. The TGF-beta 2-related decidual suppressor factor was also obtained from the decidua of synpregnant C.B.-17 severe combined immune deficiency (SCID) and pregnant SCID-BG (C57BL/6 background) mice, confirming the lack of T or B cell dependence of DSF production and the generality of production of a TGF-beta-related suppressor factor by decidua associated with successful pregnancy in mice.