Fractionation of cell mixtures using acoustic and laminar flow fields
Fractionation of cell mixtures using acoustic and laminar flow fields
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DOI：
10.1002/bit.20294
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发表时间：
2005-01-20
影响因子：
3.8
通讯作者：
Belovich, JM
中科院分区：
文献类型：
--
作者：
Kumar, M;Feke, DL;Belovich, JM
报道了一种适用于悬浮液中不同细胞群的分级分离方法。通过使悬浮液经受在薄的矩形腔室内沿正交方向传播的共振超声场和层流场来实现分离。稳定的层流使细胞悬浮液沿着腔室运输，而超声场使悬浮细胞以与其尺寸和物理性质相关的速率迁移到腔室的中间平面。位于出口附近的薄分流器将流出细胞悬浮液分成两个产物流，从而允许将对声场响应更快的细胞与响应更慢的细胞分离。对单个细胞通过腔室的轨迹进行建模表明，通过相对于声场改变流动强度，可以控制所需的分级。使用杂交瘤细胞和鼠李糖乳杆菌细胞进行概念验证实验。使用该技术可以有效地分离两个细胞群体，导致左和右产物流中的杂交瘤/乳杆菌比率（标准化为进料比率）分别为6.9+/-1.8和0.39+/-0.01（体积/体积）。声学方法是快速、有效的，并且可以连续操作，具有高度的选择性和产率，并且具有低功耗。(C)2004 Wiley Periodicals，Inc.
A fractionation method applicable to different populations of cells in a suspension is reported. The separation was accomplished by subjecting the suspension to a resonant ultrasonic field and a laminar flow field propagating in orthogonal directions within a thin, rectangular chamber. Steady, laminar flow transports the cell suspension along the chamber, while the ultrasonic field causes the suspended cells to migrate to the mid-plane of the chamber at rates related to their size and physical properties. A thin flow splitter positioned near the outlet divides the effluent cell suspension into two product streams, thereby allowing cells that respond faster to the acoustic field to be separated from those cells that respond more slowly. Modeling of the trajectories of individual cells through the chamber shows that by altering the strength of the flow relative to that of the acoustic field, the desired fractionation can be controlled. Proof-of-concept experiments were performed using hybridoma cells and Lactobacillus rhamnosus cells. The two populations of cells could be effectively separated using this technique, resulting in hybridoma/Lactobacillus ratios in the left and right product streams, normalized to the feed ratio, of 6.9+/-1.8 and 0.39+/-0.01 (vol/vol), respectively. The acoustic method is fast, efficient, and could be operated continuously with a high degree of selectivity and yield and with low power consumption. (C) 2004 Wiley Periodicals, Inc.