Expression of aquaporin-1 immunoreactivity by photoreceptor cells in the mouse retina
Expression of aquaporin-1 immunoreactivity by photoreceptor cells in the mouse retina
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DOI：
10.1016/j.neulet.2005.06.046
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发表时间：
2005-11-11
影响因子：
2.5
通讯作者：
Bringmann, A
中科院分区：
文献类型：
--
作者：
Iandiev, I;Pannicke, T;Bringmann, A
水通道蛋白水通道在维持神经组织离子和渗透稳态中起着至关重要的作用。在感觉视网膜中，水通道蛋白-4由Muller神经胶质细胞表达，主要在内层视网膜中，而水通道蛋白-1主要在外层视网膜中表达。然而，水通道蛋白-1的表达是否发生在米勒细胞或感光细胞中尚不清楚。通过对rds小鼠视网膜切片进行免疫组化染色，我们发现水通道蛋白1的免疫反应性沿着感光细胞的变性而消失。在对照组小鼠视网膜细胞的悬浮液中，感光细胞表达水通道蛋白-1免疫反应性，而Muller细胞基本上没有染色。这些数据表明，感光细胞，而不是米勒细胞，表达水通道蛋白-1在小鼠视网膜。(C)2005爱思唯尔爱尔兰有限公司保留所有权利。
Aquaporin water channels play a crucial role in the maintenance of ionic and osmotic homeostasis in the neural tissue. In the sensory retina, aquaporin-4 is expressed by Muller glial cells, predominantly in the inner retina, while aquaporin-1 is expressed mainly in the outer retina. However, it is unknown whether aquaporin-1 expression occurs in Muller cells or photoreceptor cells. By using immunohistochemical staining of retinal slices from rds mice, we show that the immumoreactivity for aquaporin-1 disappears along with the photoreceptor cell degeneration. In suspensions of dissociated retinal cells from control mice, photoreceptor cells expressed aquaporin-1 immunoreactivity while Muller cells were largely devoid of staining. The data suggest that photoreceptor cells, but not Muller cells, express aquaporin-1 in the murine retina. (C) 2005 Elsevier Ireland Ltd. All rights reserved.