Apoptosis leads to photoreceptor degeneration in inherited retinal dystrophy of RCS rats.
Apoptosis leads to photoreceptor degeneration in inherited retinal dystrophy of RCS rats.
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发表时间：
1994-05
影响因子：
4.4
通讯作者：
M. Tso;Chang Zhang;A. Abler;Cheng‐Jong Chang;F. Wong;G. Chang;T. Lam
中科院分区：
文献类型：
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作者：
M. Tso;Chang Zhang;A. Abler;Cheng‐Jong Chang;F. Wong;G. Chang;T. Lam
目的探讨皇家外科医学院（RCS）大鼠遗传性视网膜营养不良感光细胞变性的发病机制。方法采用琼脂糖凝胶电泳和原位末端标记（TUNEL）技术，观察红眼RCS（RCS-rdy-p）大鼠视网膜营养不良后的DNA断裂情况。对3至60日龄的大鼠进行了检查。结果25、30、35和40日龄动物视网膜DNA琼脂糖凝胶电泳显示梯形降解带，对应于180 - 200碱基对亚基的倍数。TUNEL结果显示随着RCS大鼠视网膜营养不良的进展，感光细胞的标记逐渐增加。结论RCS大鼠视网膜色素上皮细胞吞噬功能存在遗传缺陷，细胞凋亡是其感光细胞变性的主要机制。发病的变性出现不同的视杆细胞在不同地区的眼睛。
PURPOSE To determine the pathogenetic mechanism of photoreceptor cell degeneration in the inherited retinal dystrophy in Royal College of Surgeons (RCS) rats. METHODS The dystrophic retinas of the pink-eyed RCS (RCS-rdy-p) rats were examined for DNA fragmentation by agarose gel electrophoresis of retinal DNA and by TdT-mediated biotin-dUDP nick-end labeling (TUNEL) in paraffin sections. Rats ranging in age from 3 to 60 days were examined. RESULTS Agarose gel electrophoresis of retinal DNA isolated from animals 25, 30, 35, and 40 days old showed a ladder pattern of degradation with bands corresponding to multiples of 180 to 200 base pair subunits. TUNEL study showed increasing labeling of photoreceptor cells with progression of the retinal dystrophy of the RCS rats. CONCLUSIONS Apoptosis is the dominant mechanism of photoreceptor degeneration in the RCS rat, which has a genetic defect in the phagocytic activity of retinal pigment epithelium. The onset of the degeneration appeared to vary between rod cells in the different regions of the eye.