The Paf1 complex has functions independent of actively transcribing RNA polymerase II
The Paf1 complex has functions independent of actively transcribing RNA polymerase II
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DOI：
10.1016/s1097-2765(04)00257-6
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发表时间：
2004-05-21
期刊：
影响因子：
16
通讯作者：
Jaehning, JA
中科院分区：
文献类型：
--
作者：
Mueller, CL;Porter, SE;Jaehning, JA
酵母Paf 1复合物，最低限度地由Paf 1，Ctr 9，Cdc 73，Rtf 1和Leo 1组成，最初与RNA聚合酶II（Pol II）一起分离。Paf 1复合物成分丰富，与Pol 11共定位在启动子的染色质上和活跃转录基因的编码区中。Paf 1的缺失导致严重的表型和其他Paf 1因子的量减少，对Pol 11的丰度或染色质分布几乎没有影响，Pol 11是对转录延伸（Spt 5，Spt 16）或RNA加工（Sub 2）重要的蛋白质。Paf 1因子的缺失导致Pol II Ser 2磷酸化的减少和poly（A）尾的缩短，表明该复合物促进转录和转录后事件的连接。令人惊讶的是，Rtfl或Cdc 73的损失，几乎没有表型的后果，导致Paf 1因子从染色质的损失和Paf 1/Pol II协会的显着减少。因此，Paf 1的主要功能可以独立于Pol II的主动转录。
The yeast Paf1 complex, minimally composed of Paf1, Ctr9, Cdc73, Rtf1, and Leo1, was originally isolated in association with RNA polymerase II (Pol II). Paf1 complex components are abundant and colocalize with Pol 11 on chromatin at promoters and in the coding regions of actively transcribed genes. Loss of Paf1 results in severe phenotypes and reduced amounts of other Paf1 factors, with little effect on abundance or chromatin distribution of Pol 11, proteins important for transcriptional elongation (Spt5, Spt16), or RNA processing (Sub2). Loss of Paf1 factors causes a reduction of Pol II Ser2 phosphorylation and shortened poly(A) tails, suggesting that the complex facilitates linkage of transcriptional and posttranscriptional events. Surprisingly, loss of Rtfl or Cdc73, with little phenotypic consequence, results in loss of Paf1 factors from chromatin and a significant reduction in Paf1/Pol II association. Therefore, the major functions of Paf1 can be independent of actively transcribing Pol II.