Effect of TRPV1 gene mutation on bronchial asthma in children before and after treatment
Effect of TRPV1 gene mutation on bronchial asthma in children before and after treatment
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DOI：
10.2500/aap.2015.36.3828
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发表时间：
2015-03-01
影响因子：
2.8
通讯作者：
Zhao, Jun-Wu
中科院分区：
文献类型：
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作者：
Chen, Chuan-Liang;Li, Hong;Zhao, Jun-Wu
支气管哮喘是一种世界性的高发疾病。它不仅危害儿童的身心健康，而且给他们的家庭和社会带来了沉重的负担。然而，该疾病的触发因素和发病机制仍不清楚。本研究旨在分析儿童支气管哮喘急性发作期治疗前后TRPV 1基因突变及细胞因子的表达情况，为儿童支气管哮喘的诊断和治疗提供理论指导。采用实时荧光定量聚合酶链反应检测TRPV 1 mRNA表达水平，酶联免疫吸附法检测健康对照组和哮喘组患儿治疗前后外周静脉血总IgE水平、嗜酸性粒细胞（EOS）数、IL-4、IL-5和干扰素（IFN-γ）水平。采用Logistic回归分析儿童支气管哮喘发病的主要因素。哮喘组治疗前外周血TRPV 1 mRNA水平高于对照组（P < 0.01）。血清IL-4、IL-5、EOS水平明显高于对照组（P < 0.01），IFN-γ水平低于对照组（P < 0.01）。常规治疗后TRPV 1 mRNA水平显著升高（p < 0.01）。血清IL-4、IL-5、EOS水平较治疗前明显降低（P < 0.01），IFN-γ水平较治疗前明显升高（P < 0.01）。与治疗前相比，治疗后IgE表达水平明显下降（P < 0.01）。Logistic回归分析结果显示，TRPV 1表达水平、IL-4水平和rs 4790522位点突变是儿童支气管哮喘发病的主要危险因素。TRPV 1基因突变与儿童支气管哮喘密切相关，为儿童支气管哮喘的治疗和预后提供了理论依据。
Bronchial asthma is a worldwide disease with high incidence. It not only harms children's physical and inental health, but it also brings a heavy burden to their families as well as the society. However, the trigger and pathogenesis of the disease remain unclear. This study aimed to analyze TRPV1 gene mutation and expression of cytokines in children with acute bronchial asthma before and after treatment, thus providing theoretical guidance for the diagnosis and treatment of bronchial asthma in children. Real-time quantitative polymerase chain reaction was adopted to detect TRPV1 mRNA expression level and enzyme-linked immuno sorbent assay was used to detect the serum total IgE level, eosinophil (EOS) number, IL-4, IL-5, and interferon (IFN) gamma levels in peripheral venous blood of children in the healthy control group and asthma group before and after treatment. Logistic regression analysis was applied to analyze the most essential factor inducing bronchial asthma in children. TRPV1 mRNA level of peripheral blood in the asthma group was higher than that in the control group before treatment (p < 0.01). The IL-4, IL-5, and EOS levels in serum were markedly higher than those in the control group (p < 0.01), whereas the IFN-gamma level was lower than that in the control group (p < 0.01). After conventional treatment, TRPV1 mRNA level increased significantly (p < 0.01). The levels of serum IL-4, IL-5, and EOS were significantly lower than those before treatment (p < 0.01), whereas, IFN-gamma level was higher than that before treatment (p < 0.01). Compared with that before treatment, the expression level of IgE showed a significant decrease after treatment (p < 0.01). The results of logistic regression analysis indicated that TRPV1 expression level, IL-4 level, and rs4790522 site mutation were the main risk factors inducing bronchial asthma in children. TRPV1 gene mutation was closely related to bronchial asthma in children, which provided a theoretical basis for the treatment and prognosis of children with bronchial asthma.