Gene knockdown by morpholino-modified oligonucleotides in the zebrafish (Danio rerio) model: applications for developmental toxicology.
Gene knockdown by morpholino-modified oligonucleotides in the zebrafish (Danio rerio) model: applications for developmental toxicology.
复制标题
斑马鱼（斑马鱼）模型中吗啉代修饰寡核苷酸的基因敲除：发育毒理学的应用。
DOI：
10.1007/978-1-61779-867-2_5
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Hahn, Mark E
中科院分区：
文献类型：
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作者：
Timme-Laragy, Alicia R;Karchner, Sibel I;Hahn, Mark E
The zebrafish (Danio rerio) has long been used as a model for developmental biology, making it an excellent model to use also in developmental toxicology. The many advantages of zebrafish include their small size, prolific spawning, rapid development, and transparent embryos. They can be easily manipulated genetically through the use of transgenic technology and gene knock-down via morpholino-modified antisense oligonucleotides (MOs). Knocking down specific genes to assess their role in the response to toxicant exposure provides a way to further our knowledge of how developmental toxicants work on a molecular and mechanistic level, while establishing a relationship between these molecular events and morphological, behavioral, and/or physiological effects (i.e. phenotypic anchoring). In this chapter we address important considerations for using MOs to study developmental toxicology in zebrafish embryos and provide a protocol for their use.
