Frizzled/PCP-dependent asymmetric neuralized expression determines R3/R4 fates in the Drosophila eye
Frizzled/PCP-dependent asymmetric neuralized expression determines R3/R4 fates in the Drosophila eye
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DOI：
10.1016/j.devcel.2006.09.016
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发表时间：
2006-12-01
影响因子：
11.8
通讯作者：
Miodzik, Marek
中科院分区：
文献类型：
--
作者：
del Alamo, David;Miodzik, Marek
平面细胞极性（PCP）是许多上皮细胞的共同特征，反映在上皮平面内的细胞组织中。在果蝇眼中，卷曲（Fz）/PCP信号通过调节R4中的Notch激活诱导R3/R4光感受器的细胞命运特化。除了R3中的DI上调之外，Fz/PCP信号传导如何在这种情况下调节Notch的机制尚不清楚。我们证明，E3-泛素连接酶神经化（Neur），所需的DI-N信号，是不对称的R3/R4对内表达。它在R3中是必需的，其中它也以Fz/PCP依赖性方式上调。与DI的情况一样，R4中的N活性进一步抑制neur表达，从而加强了不对称性。我们证明神经元的不对称性是指导正确的R3/R4规范。我们的数据表明，在R3中Fz/PCP依赖的Neur表达确保了在R3/R4特化期间DI-N信号传导的正确方向性。
Planar cell polarity (PCP) is a common feature in many epithelia, reflected in cellular organization within the plane of an epithelium. In the Drosophila eye, Frizzled (Fz)/PCP signaling induces cell-fate specification of the R3/R4 photoreceptors through regulation of Notch activation in R4. Except for DI upregulation in R3, the mechanism of how Fz/PCP signaling regulates Notch in this context is not understood. We demonstrate that the E3-ubiquitin ligase Neuralized (Neur), required for DI-N signaling, is asymmetrically expressed within the R3/R4 pair. It is required in R3, where it is also upregulated in a Fz/PCP-dependent manner. As is the case for DI, N activity in R4 further represses neur expression, thus, reinforcing the asymmetry. We demonstrate that Neur asymmetry is instructive in correct R3/R4 specification. Our data indicate that Fz/PCP-dependent Neur expression in R3 ensures the proper directionality of DI-N signaling during R3/R4 specification.