TCDD and corticosterone on testicular steroidogenesis and antioxidant system of epididymal sperm in rats
TCDD and corticosterone on testicular steroidogenesis and antioxidant system of epididymal sperm in rats
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DOI：
10.1177/0748233713475501
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发表时间：
2015-09-01
影响因子：
1.9
通讯作者：
Latha, P.
中科院分区：
文献类型：
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作者：
Dhanabalan, S.;Mathur, P. P.;Latha, P.
2，3，7，8-四氯二苯并二恶英（TCDD）是一种内分泌干扰物，可引起雄性生殖毒性。已经发现糖皮质激素影响TCDD的代谢途径。压力影响男性生殖功能，其特征是体内糖皮质激素水平和活性增加。本研究旨在探讨在体内皮质酮水平升高的情况下，TCDD对睾丸类固醇生成和精子抗氧化系统的影响。成年雄性大鼠接受TCDD（100 ng/kg bw/天）或皮质酮（3 mg/kg bw/天）或两者共15天的治疗。用TCDD或皮质酮治疗发现抑制类固醇生成急性调节蛋白和雄激素结合蛋白的水平，并降低睾丸中类固醇生成酶的活性，同时增加腹侧前列腺，精囊和附睾精子的氧化应激。在大鼠治疗与TCDD和皮质酮，睾丸类固醇生成的抑制和腹侧前列腺，精囊和附睾精子中观察到的氧化应激的增加是显着的相比，TCDD单独治疗的大鼠。Fas和FasL蛋白的水平也增加了大鼠进行TCDD或皮质酮治疗。在用这两种化合物处理的大鼠中，观察到的睾丸Fas和FasL水平的增加与单独用TCDD处理的大鼠相比是显著的。体内糖皮质激素水平升高时，TCDD对睾丸类固醇生成和附睾精子抗氧化系统的影响可能增强。
2,3,7,8-Tetrachloro dibenzo-p-dioxin (TCDD), an endocrine-disrupting environmental pollutant, has been found to cause male reproductive toxicity. Glucocorticoids have been found to influence the metabolic pathway of TCDD. Stress, which affects the male reproductive function, is marked by an increase in the level and activity of glucocorticoids in the body. The present study was carried out to understand the effect of TCDD on testicular steroidogenesis and sperm antioxidant system under the influence of increased level of corticosterone in the body. Adult male rats were treated with either TCDD (100 ng/kg bw/ day) or corticosterone (3mg/kgbw/day) or both for 15days. Treatment with either TCDD or corticosterone was found to suppress the levels of steroidogenic acute regulatory protein and androgen-binding protein and reduce the activities of steroidogenic enzymes in testis while increasing oxidative stress in ventral prostate, seminal vesicles and epididymal sperm. In rats treated with both TCDD and corticosterone, the suppression of testicular steroidogenesis and increase in oxidative stress observed in ventral prostate, seminal vesicles and epididymal sperm were significant as compared to TCDD alone treated rats. The levels of Fas and FasL proteins were also increased in rats subjected to either TCDD or corticosterone treatment. In rats treated with both compounds, the increase observed in testicular levels of Fas and FasL was significant as compared to TCDD alone treated rats. Effect of TCDD on testicular steroidogenesis and antioxidant system of epididymal sperm may get enhanced under increased level of glucocorticoids in the body.