Fibroblasts play a potential role in bone destruction via osteopontin related caldesmon expression and polymerization in human non-functioning pituitary adenomas.
Fibroblasts play a potential role in bone destruction via osteopontin related caldesmon expression and polymerization in human non-functioning pituitary adenomas.
复制标题
成纤维细胞通过骨桥蛋白相关的钙结合蛋白表达和聚合在人类无功能垂体腺瘤中的骨破坏中发挥潜在作用
DOI：
10.1038/s41598-017-17679-2
复制
发表时间：
2017-12-13
影响因子：
4.6
通讯作者：
Liu ZX
中科院分区：
文献类型：
--
作者：
Zhang LY;Ge XL;Li Z;Tang YJ;Xiong YY;Li XJ;Liu JF;Wanggou SY;Li CT;Yang K;Chen X;Hu ZL;Liu YS;Liu ZX
无功能垂体腺瘤是最常见的垂体肿瘤。为了指导nfpa的临床诊断和治疗，阐明侵袭性nfpa破坏骨的机制是必要的。在本研究中，我们研究了从有或没有骨破坏的nfpa临床标本中分离的成纤维细胞的差异蛋白质组学。蛋白质组学分析显示，从骨破坏nfpa中分离的成纤维细胞（bd - nfpa）和从非骨破坏nfpa中分离的成纤维细胞（nbd - nfpa）中分离的一组与细胞骨架组织相关的分子，包括caldesmon，表达差异。分泌蛋白分析发现，骨桥蛋白在bd - nfpa成纤维细胞中显著上调。此外，对nfpa临床样本进行免疫组化染色发现，在pd - nfpa中，基质细胞中caldesmon的表达以及肿瘤细胞和基质中骨桥蛋白的表达均显著升高。综上所述，我们的研究结果表明，NFPA细胞和周围成纤维细胞分泌的骨桥蛋白可能会改变成纤维细胞中caldesmon的表达和聚合，这可能有助于NFPA患者的骨破坏。
Non-functioning pituitary adenomas (NFPAs) are the most frequent pituitary tumors. The elucidation of the mechanisms of aggressive NFPAs in bone destruction is required in order to guide the clinical diagnosis and treatment of NFPAs. In the present study, we investigated the differential proteomics of fibroblasts isolated from clinical specimens of NFPAs with or without bone destruction. Proteomic analysis revealed a group of molecules associated with cytoskeleton organization, including caldesmon, were differentially expressed between fibroblasts isolated from bone destruction NFPAs (BD-NFPAs) and fibroblasts isolated from non-bone destruction NFPAs (NBD-NFPAs). The secreted proteins analysis found that osteopontin was significantly upregulated in BD-NFPAs fibroblasts. Furthermore, immunohistochemical staining of the NFPAs clinical samples showed that the expression of caldesmon in stromal cells and the expression of osteopontin in both tumor cells and stroma were significantly increased in BD-NFPAs. Taken together, our results indicate a possible way that osteopontin secreted from both NFPA cells and surrounding fibroblasts modify caldesmon expression and polymerization in fibroblasts, which may contribute to bone destruction in NFPA patients.
登录
查看更多内容
影响因子：
11.2
作者：
Gao D;Vahdat LT;Wong S;Chang JC;Mittal V
通讯作者：
Mittal V
影响因子：
8.8
作者：
Thoms, J. W.;Dal Pra, A.;Anborgh, P. H.;Christensen, E.;Fleshner, N.;Menard, C.;Chadwick, K.;Milosevic, M.;Catton, C.;Pintilie, M.;Chambers, A. F.;Bristow, R. G.
通讯作者：
Bristow, R. G.
影响因子：
12.8
作者：
Dickerson TJ;Suzuki E;Stanecki C;Shin HS;Qui H;Adamopoulos IE
通讯作者：
Adamopoulos IE
影响因子：
4.8
作者：
Topalovski, Mary;Hagopian, Michelle;Brekken, Rolf A.
通讯作者：
Brekken, Rolf A.
影响因子：
4.8
作者：
Tanabe, Natsuko;Wheal, Benjamin D.;Dixon, S. Jeffrey
通讯作者：
Dixon, S. Jeffrey