A Facile and Sensitive DNA Sensing of Harmful Algal Blooms Based on Graphene Oxide Nanosheets
A Facile and Sensitive DNA Sensing of Harmful Algal Blooms Based on Graphene Oxide Nanosheets
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基于氧化石墨烯纳米片的有害藻华的简便、灵敏的 DNA 传感
DOI：
10.1007/s10126-020-09971-6
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发表时间：
2020-05-02
影响因子：
3
通讯作者：
Han, Lin
中科院分区：
文献类型：
--
作者：
Qiang, Le;Zhang, Yu;Han, Lin
基因检测在生物学、生物医学工程、临床、环境、海洋等领域有着重要的应用。快速、灵敏和选择性地识别特定基因在实际应用中是必不可少的。本研究建立了一种简便、灵敏的DNA传感平台，用于赤潮病原之一赤潮异弯藻的特异性定量检测。通过使用化学合成的氧化石墨烯（GO）纳米片实现快速和灵敏的检测。根据有害藻类的特异性DNA片段设计探针DNA，并用荧光分子FAM（荧光素基染料）标记。制备GO纳米片溶液，其中FAM标记的探针和GO纳米片之间的强相互作用使它们保持紧密接近，促进GO纳米片对荧光团的荧光猝灭。在互补靶DNA的存在下，FAM标记的DNA探针和靶DNA杂交并从GO纳米片表面解吸附，导致荧光团荧光恢复。通过在约520 nm处的荧光强度和480 nm的发射波长分析靶DNA片段的浓度。灵敏的检测平台实现了对赤潮异弯藻特异基因的稳定检测。我们的基于GO纳米片的DNA传感平台可以快速灵敏地检测痕量DNA，并能够定量识别有害藻类，这在实时监测海洋环境中的赤潮生成动态方面具有很好的应用前景，可以进行有效的控制，特别是与海水养殖有关。
Gene detection has important applications in biology, biomedical engineering, clinical, environmental, and marine fields. Rapid, sensitive, and selective recognition of specific genes is essential in practical applications. In this study, we describe a facile and sensitive DNA sensing platform for specific and quantitative detection ofHeterosigma akashiwo, which is one of the causative agents of red tides. Fast and sensitive detection is achieved by using chemically synthesized graphene oxide (GO) nanosheets. Probe DNA is designed according to the specific DNA fragments of harmful algae and labeled with fluorescent molecules FAM (fluorescein-based dye). GO nanosheet solution is made, in which the strong interaction between FAM-labeled probe and GO nanosheets keeps them in close proximity, facilitating the fluorescence quenching of the fluorophore by GO nanosheets. In the presence of a complementary target DNA, the FAM-labeled DNA probe and the target DNA hybridize and desorb from the surface of GO nanosheets, resulting in restoration of fluorophore fluorescence. The concentration of target DNA fragments is analyzed by the fluorescence intensity at ~ 520 nm with emission wavelength of 480 nm. The sensitive detecting platform achieved stable measurement of 1 pM specific genes fromHeterosigma akashiwo. Our GO nanosheet-based DNA-sensing platform performs fast and sensitive detection of trace amount of DNA, and enables quantitative recognition of harmful algae, which has promising applications in real-time monitoring in the marine environment of red tide generative dynamics, allowing effective control, particularly in relation to marine aquaculture.