Differential effects of maternal stress on circulating levels of corticosterone, progesterone, and testosterone in male and female rat fetuses and their mothers.
Differential effects of maternal stress on circulating levels of corticosterone, progesterone, and testosterone in male and female rat fetuses and their mothers.
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DOI：
10.1210/endo-114-5-1635
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发表时间：
1984-05
期刊：
影响因子：
4.8
通讯作者：
I. L. Ward;J. Weisz
中科院分区：
文献类型：
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作者：
I. L. Ward;J. Weisz
用放射免疫法测定了应激孕鼠和对照组孕鼠及其雌雄胎儿在妊娠第17、18、19、21天和出生当天血浆中睾酮、孕酮和皮质酮的滴度。应激疗法(从怀孕14-21天起，每天在强光下进行三次45分钟的束缚)会导致雄性后代的男性化和去女性化。在怀孕后第17天至第18天(PC)，男女胎儿的皮质酮滴度都急剧上升，一直维持到第19天，然后下降。这一模式类似于对照组男性胎儿的睾酮水平，后者的睾酮水平在第17天到18天之间也急剧上升。在应激过程中，从母亲(血清)和他们的男性和女性胎儿(血浆)获得的样本中皮质酮滴度都升高。在应激过程结束后75-165分钟，从胎儿采集的样本中皮质酮水平升高不再明显。只有男性胎儿的睾丸激素滴度会因应激而改变。应激结束后75-165min，雄鼠的睾酮水平在第17天升高，而在第18天和第19天没有出现与正常男性相同的峰值。无论是母亲还是胎儿，孕酮滴度都不受压力的影响。在应激组和对照组，孕酮浓度在男性和女性胎儿中是相同的，母亲的孕酮浓度高于胎儿，在第19天到第21天之间，胎儿和母亲的孕酮浓度都下降了。因此，压力只对睾丸激素有持续的影响，而且只在男性身上观察到。
Testosterone, progesterone, and corticosterone titers were measured by RIA in plasma of stressed and control pregnant rats and their male and female fetuses on days 17, 18, 19, and 21 of gestation and on the day of birth. The regimen of stress used (three 45-min periods of restraint under intense illumination daily from days 14-21 of pregnancy) causes failure of masculinization and defeminization of behavioral potentials in male offspring. In fetuses of both sexes, corticosterone titers increased sharply between days 17 and 18 postconception (pc) to a peak that was maintained through day 19 and then declined. This pattern resembled that obtained for testosterone in control male fetuses in which the levels of testosterone also rose sharply between days 17 and 18 pc. Corticosterone titers were elevated in samples obtained during the middle of the stress session from both the mothers (serum) and their male and female fetuses (plasma). Increased corticosterone levels were no longer evident in samples obtained from fetuses 75-165 min after the end of a stress session. Testosterone titers were altered by stress only in male fetuses. Their testosterone levels were elevated on day 17 pc, and the surge on days 18 and 19 pc, characteristic of control males, was absent in samples obtained 75-165 min after termination of stress. Progesterone titers were not affected by stress in either mothers or their fetuses. In both stressed and control groups, progesterone concentrations were identical in male and female fetuses, were higher in mothers than in fetuses, and declined in both fetuses and mothers between days 19 and 21 pc. Thus, a persistent effect of stress was observed only on testosterone and only in males.