KINETICS OF RED-BLOOD-CELL PASSAGE THROUGH INTERENDOTHELIAL SLITS INTO VENOUS SINUSES IN RAT SPLEEN, ANALYZED BY INVIVO MICROSCOPY
KINETICS OF RED-BLOOD-CELL PASSAGE THROUGH INTERENDOTHELIAL SLITS INTO VENOUS SINUSES IN RAT SPLEEN, ANALYZED BY INVIVO MICROSCOPY
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DOI：
10.1016/0026-2862(87)90011-2
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发表时间：
1987-01-01
影响因子：
3.1
通讯作者：
GROOM, AC
中科院分区：
文献类型：
--
作者：
MACDONALD, IC;RAGAN, DM;GROOM, AC
通过视频记录活体显微镜图像，获得了红细胞穿过大鼠脾静脉窦壁内皮间缝（IES）的连续显微照片。通过慢动作回放录音来分析红细胞通过的动力学。倒置显微镜和水冷光纤光源的倾斜照明是获得足够质量的分析图像的关键要素。红细胞通过 IES 的方向始终是从红髓的网状间隙进入静脉窦。流经单个 IES 的红细胞流量以短暂的不连续突发形式发生，由零或接近零流量的周期分隔开。在正常松弛的脾脏中分析的红细胞流过六个 IES 的平均速率范围为 1.4 至 9.1 个细胞/15 秒，研究的红细胞总数为 1523 个，总观察时间为 98 分钟。最大瞬时速率为 10 个红细胞/秒。 RBC 传输时间范围为 0.02 至 60.5 秒，即使对于单个 IES 也是如此；分布高度倾斜：中位数为 0.23 秒。平均 1.7 秒对红细胞流同时通过两个紧密相邻的 IES 进行 30 分钟的分析表明，大多数突发是异步的。结果表明 IES 口径的变化是造成观察到的流动模式的主要原因。据估计，总 IES 蛋白中只有 19% 在解剖学上实际上允许红细胞在任何 5 分钟内通过。
Sequential photomicrographs of RBCs passing through interendothelial slits (IES) in walls of venous sinuses in rat spleen were obtained by video recording in vivo microscopic views. Kinetics of RBC passage were analyzed by slow-motion playback of recordings. An inverted microscope and oblique lighting from a water-cooled fiber optic light source were key elements in obtaining images of sufficient quality for analysis. The direction of RBC passage through IES was, invariably, from reticular spaces of the red pulp into venous sinuses. RBC flow through an individual IES occurred as brief discontinuous bursts, separated by periods of zero, or near zero, flow. Mean rates of RBC flow through six IES analyzed in normal relaxed spleen ranged from 1.4 to 9.1 cells/15 sec, the total RBCs studied being 1523 and the total combined period of observation 98 min. The maximum instantaneous rate was 10 RBCs/sec. RBC transit times ranged from 0.02 to 60.5 sec, even for a single IES; the distribution was highly skewed: median 0.23 sec. mean 1.7 sec. Analysis of RBC flow through two closely adjacent IES simultaneously, for 30 min, showed that most bursts were asynchronous. The results indicate that changes in caliber of IES are primarily responsible for the observed pattern of flow. It was estimated that only 19% of the total IES protein anatomically actually allowed passage of RBCs during any 5-min period.