Electrochemical recycling of gold nanofibrous membrane as an enzyme immobilizing carrier
Electrochemical recycling of gold nanofibrous membrane as an enzyme immobilizing carrier
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DOI：
10.1016/j.cej.2015.06.029
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发表时间：
2015-11
影响因子：
15.1
通讯作者：
Koichi Sawada;S. Sakai;M. Taya
中科院分区：
文献类型：
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作者：
Koichi Sawada;S. Sakai;M. Taya
金纳米纤维膜是一种酶固定化载体，可以在连续流动反应器中通过简单的电化学处理进行现场回收。采用聚丙烯腈/金盐溶液静电纺丝、化学镀金的方法制备了聚丙烯腈/金盐复合膜。以漆酶为模型酶，通过端氨基自组装单分子膜的形成和戊二醛处理相结合的方法将漆酶固定在金膜表面。电化学脱附2，2′-azinobis(3-ethylbenzothiazoline-6-sulfonate)(ABTS)底物后，酶活力下降了98%，并用X射线光电子能谱对其表面进行了表征，证实了中性pH下酶的脱附。在膜上重新固定化后酶活恢复到90%，解吸后酶活降至1.0%。这些结果表明，金纳米纤维膜作为酶固定化载体的回收是可能的。将漆酶固定化膜用于流通式反应器，膜上固定化和再固定化酶的活性分别为运行7d后初始活性的62.7%和58.5%。
A gold nanofibrous membrane was shown to be an enzyme-immobilizing carrier that can be recycled on-site in a continuous flow-through reactor using a simple electrochemical treatment. The membrane was prepared by electrospinning a polyacrylonitrile/gold salt solution, followed by electroless gold plating. Laccase, used as a model enzyme, was immobilized on the gold membrane surface by a combination of formation of amino-terminated self-assembled monolayers and glutaraldehyde treatment. Enzyme desorption at neutral pH was confirmed by a 98% decrease in enzymatic activity, based on oxidation of a 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) substrate, after electrochemical treatment for desorption, and surface characterization using X-ray photoelectron spectroscopy. The enzymatic activity after re-immobilization on the membrane was restored to 90%, and decreased to 1.0% after redesorption. These results showed that recycling of the gold nanofibrous membrane as an enzyme-immobilizing carrier was possible. The laccase-immobilized membrane was used in a flow-through reactor; the activities of the immobilized and re-immobilized enzymes on the membrane were 62.7% and 58.5% of the respective initial activities after operation for 7 d.