An estimation of the minimum number of SSR alleles needed to reveal genetic relationships in wheat varieties. I. Information from large-scale planted varieties and cornerstone breeding parents in Chinese wheat improvement and production
An estimation of the minimum number of SSR alleles needed to reveal genetic relationships in wheat varieties. I. Information from large-scale planted varieties and cornerstone breeding parents in Chinese wheat improvement and production
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DOI：
10.1007/s00122-002-1016-z
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发表时间：
2002-08
影响因子：
5.4
通讯作者：
Zhang Xy;Li Cw;Lanfen Wang;Wang Hm;You Gx;Yuchen Dong
中科院分区：
文献类型：
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作者：
Zhang Xy;Li Cw;Lanfen Wang;Wang Hm;You Gx;Yuchen Dong
利用SSR标记对43个小麦品种的亲缘关系进行了分析。所用材料包括1980年以前使用的14个基础育种亲本和29个目前在中国使用的其他大面积种植品种。共扩增出501个等位基因，其中，A基因组166个，B基因组174个，D基因组161个。利用DICE系数估计遗传相似性，并利用UPGMA法构建系统树。利用501个等位基因构建的树状图成功区分了所有基础育种亲本和大面积种植的品种，树状图与这些品种的系谱基本一致。501个等位基因的遗传距离矩阵与450个等位基因的遗传距离矩阵的相关系数为0.99。等位基因随机样本间的相关系数表明，检测普通小麦品种间的遗传关系需要350 ~ 400个等位基因。基于580个随机等位基因的遗传相似性矩阵与501和400个随机等位基因的遗传相似性矩阵的相关系数分别为0.96和0.94。然而，基于501个等位基因的矩阵与基于独立位于A、B或D基因组的标记的矩阵之间存在显著差异。501个等位基因的遗传距离矩阵与A、BordD基因组内等位基因的相关系数分别为0.77、0.76和0.67。遗传相似性的估计应该基于所有基因组的数据，而不是任何一个基因组。
The genetic relationships of 43 wheat varieties were analyzed with SSR markers. The materials employed included 14 cornerstone breeding parents used before 1980 and another 29 other large-scale planted varieties currently in use in China. A total of 501 different alleles were amplified, including 166 alleles of theAgenome, 174 of theBgenome and 161 of theDgenome. Data obtained were used to estimate genetic similarity using the DICE coefficient, and dendrograms were constructed using the UPGMA method. The dendrogram with 501 alleles successfully differentiated all the cornerstone breeding parents and the large-scale planted varieties, and the dendogram tree was basically consistent with the pedigrees of these varieties. The correlation coefficient between the genetic distance matrix of 501 alleles and that of 450 was 0.99. Correlation coefficients among random samples of alleles suggested that 350 to 400 alleles were needed to detect genetic relationships among common wheat varieties. Correlation coefficients of a genetic similarity matrix based on 580, and those of 501 and 400, random alleles were 0.96 and 0.94, respectively. However, there were marked differences between the matrix based on the 501 alleles and those based on markers located on theA-,B- orD-genome independently. The correlation coefficients between the genetic distance matrix of 501 alleles and alleles withinA,BorDgenomes were 0.77, 0.76 and 0.67. The estimation of genetic similarity should be based on data from all genomes rather than any one genome.