The chicken immediate-early gene ZENK is expressed in the medio-rostral neostriatum/hyperstriatum ventrale, a brain region involved in acoustic imprinting, and is up-regulated after exposure to an auditory stimulus
The chicken immediate-early gene ZENK is expressed in the medio-rostral neostriatum/hyperstriatum ventrale, a brain region involved in acoustic imprinting, and is up-regulated after exposure to an auditory stimulus
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DOI：
10.1016/j.neuroscience.2004.10.015
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发表时间：
2005-12
期刊：
影响因子：
3.3
通讯作者：
C. Thode;J. Bock;K. Braun;M. Darlison
中科院分区：
文献类型：
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作者：
C. Thode;J. Bock;K. Braun;M. Darlison
zenk（哺乳动物基因zif-268、zf-1、ngfi-a和krox-24的鸟类直向同源基因的首字母缩写）作为神经元活动的标志物，在对鸣禽的研究中被广泛使用，以揭示与获得、感知和产生歌曲相关的记忆过程中所涉及的前脑结构。听觉诱导的该基因在脑中的表达，最近也报道了家鸡（Gallus gallus arteriticus）和日本鹌鹑（Coturnix coturnix japonica）。虽然描述了鹌鹑zenk表达的解剖学分布，但没有报道鸡的相应数据。因此，我们使用原位杂交来定位1天大小鸡大脑中编码zenk基因（我们称之为ZENK）产物的mRNA。我们证明，这种转录本是存在于一些前脑结构，包括中喙新纹状体/腹侧纹状体（MNH），一个区域，已强烈牵连在听觉印记（这是一种形式的识别记忆），和字段L，哺乳动物听觉皮层的鸟类模拟。由于这种模式的基因表达，我们比较了ZENK mRNA的水平在小鸡已经进行了30分钟的声学印迹范例，并在未经训练的控制。我们的结果显示，在MNH和Field L以及两个前脑半球中，ZENK mRNA水平显著增加（P≤0.05）;在外纹状体（视觉投射区）中没有观察到增加。所获得的数据牵连的即时早期基因，zenk，在听觉印记，这是一个既定的青少年学习模式。此外，我们的研究结果表明，ZENK mRNA可用作MNH的分子标记物，MNH是一个难以在解剖学和组织化学上描绘的区域。
The immediate-early gene zenk (an acronym for the avian orthologue of the mammalian genes zif-268, egr-1, ngfi-a and krox-24) has been extensively employed, in studies on oscine birds, as a marker of neuronal activity to reveal forebrain structures that are involved in the memory processes associated with the acquisition, perception and production of song. Audition-induced expression of this gene, in brain, has also recently been reported for the domestic chicken (Gallus gallus domesticus) and the Japanese quail (Coturnix coturnix japonica). Whilst the anatomical distribution of zenk expression was described for the quail, corresponding data for the chicken were not reported. We have, therefore, used in situ hybridisation to localise the mRNA that encodes the product of the zenk gene (which we call ZENK) within the brain of the 1-day-old chick. We demonstrate that this transcript is present in a number of forebrain structures including the medio-rostral neostriatum/hyperstriatum ventrale (MNH), a region that has been strongly implicated in auditory imprinting (which is a form of recognition memory), and Field L, the avian analog of the mammalian auditory cortex. Because of this pattern of gene expression, we have compared the level of the ZENK mRNA in chicks that have been subjected to a 30-min acoustic imprinting paradigm and in untrained controls. Our results reveal a significant increase (P≤0.05) in the level of the ZENK mRNA in MNH and Field L, and in the two forebrain hemispheres; no increase was seen in the ectostriatum, which is a visual projection area. The data obtained implicate the immediate-early gene, zenk, in auditory imprinting, which is an established model of juvenile learning. In addition, our results indicate that the ZENK mRNA may be used as a molecular marker for MNH, a region that is difficult to anatomically and histochemically delineate.