Differential cytokine levels and immunoreactivities against Mycobacterium tuberculosis antigens between tuberculous and malignant effusions
Differential cytokine levels and immunoreactivities against Mycobacterium tuberculosis antigens between tuberculous and malignant effusions
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DOI：
10.1016/j.rmed.2007.08.016
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发表时间：
2008-02-01
影响因子：
4.3
通讯作者：
Jo, Eun-Kyeong
中科院分区：
文献类型：
--
作者：
Yang, Chul-Su;Lee, Ji-Sook;Jo, Eun-Kyeong
结核性胸膜炎（TBP）和恶性胸膜炎（MP）的免疫学重要因素的鉴定，以提高对淋巴细胞主导型胸膜炎的两种主要病因的鉴别诊断。本研究评估了胸腔积液中宿主（细胞因子和趋化因子）和病原体（分枝杆菌蛋白）因子的免疫反应性和潜在诊断价值。我们收集了41例肺癌所致MP和81例TBP患者的积液样本。9种细胞因子和趋化因子(白细胞介素(IL)- 12p40、干扰素（IFN)- γ、肿瘤坏死因子(TNF)- α、IL-6、IL-10、CXCL8/IL-8、CXCL10/IP-10、CCL3/MIP-1 α和CCL4/MIP-1 β）的浓度和抗体（IgG、IgM和IgA）对5种结核分枝杆菌抗原(早期分泌抗原靶点(ESAT)-6、30-kDa、MTB12、38-kDa、采用酶联免疫吸附试验（ELISA）测定胸膜液中肝素结合血凝素（HBHA）的含量。在logistic回归中，IFN-gamma(优势比，7.178；95%可信区间（CI）， 2.258-22.817；p = 0.001), IL-12 p40(优势比，11.037;95% CI, 3.38 ~ 36.037
Much effort has been devoted to the identification of immunologically important factors in tuberculous pleurisy (TBP) and malignant pleurisy (MP) to improve the differential diagnosis of the two major causes of lymphocyte-dominant pleurisy. This study evaluated the immunoreactivity and potential diagnostic utility of both host (cytokines and chemokines) and pathogen (mycobacteriat proteins) factors in pleural effusions. Effusion samples were collected from 41 patients with MP caused by lung cancer and from 81 patients with TBP. The concentrations of nine cytokines and chemokines (interleukin (IL)-12 p40, interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha, IL-6, IL-10, CXCL8/IL-8, CXCL10/IP-10, CCL3/MIP-1 alpha, and CCL4/MIP-1 beta) and antibody responses (IgG, IgM, and IgA) against five Mycobacterium tuberculosis antigens (early secreted antigenic target (ESAT)-6, 30-kDa, MTB12, 38-kDa, and a heparin-binding hemagglutinin (HBHA)) were determined in pleural fluids using enzyme-linked immunosorbent assays (ELISA). In the logistic regression, IFN-gamma (odds ratio, 7.178; 95% confidence interval (CI), 2.258-22.817; p = 0.001), IL-12 p40 (odds ratio, 11.037; 95% CI, 3.38-36.037; p