Vimentin expression is associated with decreased survival in gastric cancer
Vimentin expression is associated with decreased survival in gastric cancer
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DOI：
10.3892/or.2011.1185
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发表时间：
2011-05-01
期刊：
影响因子：
4.2
通讯作者：
Sugihara, Kenichi
中科院分区：
文献类型：
--
作者：
Otsuki, Sho;Inokuchi, Mikito;Sugihara, Kenichi
富含间质组织的胃癌，如弥漫型疾病，预后较差。在一些癌症中，间充质生成因子波形蛋白的表达与生存不良有关。在上皮-间充质转化(EMT)后，观察到波形蛋白等间充质标志物的表达，这是一种重要的初始行为变化，与肿瘤细胞的黏附和迁移特性有关，是局部肿瘤侵袭所必需的。EMT的一个特点是E-钙粘蛋白的丢失。EMT诱导调节因子，包括SIPI、Slug和Twist，通过与E-钙粘蛋白启动子相互作用抑制E-钙粘蛋白的转录。我们用实时定量逆转录聚合酶链式反应检测了波形蛋白和EMT相关基因，包括SIPI、Slug和Twist在冷冻的癌组织和正常组织中的表达。肿瘤标本取自106例行胃切除术的胃腺癌患者。研究这些基因的表达与临床病理因素和预后的关系。根据Lauren‘s分类，弥漫型患者的Vimentin mRNA水平明显高于肠型患者(p=0.048)，复发或远处转移患者的Vimentin mRNA水平明显升高(p=0.049)。然而，免疫组织化学仅在癌间质中检测到波形蛋白。Twist mRNA的表达与肿瘤深度(p=0.042)和晚期肿瘤分期(I-II vs III-IV，p=0.030)显著相关。E-钙粘素免疫组织化学表达与Lauren‘s组织学类型显著相关(P
Gastric cancer rich in stromal tissue, such as diffuse-type disease, carries a poor prognosis. In some cancers, expression of vimentin, a mesenchymal maker, is associated with poor survival. The expression of mesenchymal markers such as vimentin is observed after epithelial-mesenchymal transition (EMT), an important initial behavioral change related to the adhesion and migration properties of tumor cells that is required for local tumor invasion. A hallmark of EMT is the loss of E-cadherin. EMT-inducing regulators, including SIP I, Slug, and Twist, repress E-cadherin transcription by interacting with E-cadherin promoter. We investigated the expression of vimentin and EMT-related genes, including SIP I, Slug, and Twist, in frozen cancer tissues and normal tissues by real-time quantitative reverse-transcriptase polymerase chain reaction. Tumor samples were obtained from 106 patients with gastric adenocarcinomas who underwent a gastrectomy. The relation of the expression of these genes to clinicopathological factors and outcomes was studied. Vimentin mRNA was significantly higher in diffuse type compared to intestinal type according to Lauren's classification (p=0.048) and was significantly elevated in patients with recurrent or distant metastatic disease (p=0.049). Immunohistochemically, however, vimentin was detected only in cancer stroma. Twist mRNA expression significantly correlated with tumor depth (p=0.042) and advanced tumor stage (I-II vs. III-IV, p=0.030). E-cadherin immunohistochemical expression was significantly associated with Lauren's histopathological type (p