Localization of 5S RNA Genes on Drosophila Chromosomes by RNA-DNA Hybridization
Localization of 5S RNA Genes on Drosophila Chromosomes by RNA-DNA Hybridization
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RNA-DNA 杂交对果蝇染色体上 5S RNA 基因的定位
DOI：
10.1126/science.170.3958.639
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发表时间：
1970
期刊：
影响因子：
56.9
通讯作者：
D. Steffensen
中科院分区：
文献类型：
--
作者：
D. E. Wimber;D. Steffensen
用[3H]尿苷接触4 d的黑腹果蝇幼虫制备了具有高比活性的[3H]RNA。将纯化的5S RNA与在甲酰胺中变性的多烯染色体DNA进行退火处理。5S RNA基因位于2号染色体右臂56E-F区。这种定位是通过放射自显像确定的，其中[3H]RNA和DNA杂交的放射性仅限于56E-F段。
[3H]RNA with a high specific activity was prepared from larvae of Drosophila melanogaster grown 4 days in contact with [3H]uridine. Purified tritiated 5S RNA was annealed to the DNA of polytene chromosomes, which had been denatured in formamide. The 5S RNA genes are placed within the region 56E-F of the right arm of chromosome 2. This localization was determined from autoradiographs, where the radioactivity from hybrids of [3H]RNA and DNA was confined to the 56E-F segment.