Identification and Characterization of FabA from the Type II Fatty Acid Synthase of Streptomyces coelicolor.
Identification and Characterization of FabA from the Type II Fatty Acid Synthase of Streptomyces coelicolor.
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天蓝色链霉菌 II 型脂肪酸合酶 FabA 的鉴定和表征。
DOI：
10.1021/acs.jnatprod.5b00560
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发表时间：
2016
影响因子：
5.1
通讯作者：
Reynolds,KevinA
中科院分区：
文献类型：
--
作者：
Singh,Renu;Reynolds,KevinA
FabA被认为是天蓝色链霉菌脂肪酸合成和十一烷基普地吉宁合成中链延长脱水步骤的催化剂。分析了S.在大肠杆菌基因组中发现了一个abA基因（SCO 4636-SCO 4637，编码异二聚体3-羟酰基-ACP脱氢酶）。在此，我们报告了相应的基因产物的鉴定和表征。动力学分析表明，FabA能够利用各种链长的直链和支链3-羟基酰基-NAC底物。此外，FabA不区分来自初级代谢和次级代谢的酰基载体蛋白（ACP）。这些数据提供了第一个实验证据，FabA具有3-羟酰基-ACP脱氢酶活性，并加工两种生物合成途径的中间体。
FabA is proposed to catalyze the dehydration step of chain elongation in fatty acid and undecylprodiginine biosynthesis inStreptomyces coelicolor. Analysis of theS. coelicolorgenome has revealed afabAgene (SCO4636-SCO4637, encoding a heterodimer 3-hydroxyacyl-ACP dehydratase). Herein, we report the identification and characterization of the corresponding gene products. Kinetic analysis has demonstrated that FabA is capable of utilizing various chain lengths of straight- and branched-chain 3-hydroxyacyl-NAC substrates. Additionally, FabA does not discriminate between acyl carrier proteins (ACPs) from primary and secondary metabolism. These data provide the first experimental evidence that FabA has 3-hydroxyacyl-ACP dehydratase activity and processes intermediates for both biosynthetic pathways.