Delayed leaf greening involves a major shift in the expression of cytosolic and mitochondrial ribosomes to plastid ribosomes in the highly phosphorus-use-efficient Hakea prostrata (Proteaceae)
Delayed leaf greening involves a major shift in the expression of cytosolic and mitochondrial ribosomes to plastid ribosomes in the highly phosphorus-use-efficient Hakea prostrata (Proteaceae)
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DOI：
10.1007/s11104-023-06275-1
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发表时间：
2023-09
期刊：
影响因子：
4.9
通讯作者：
Toby Bird;B. J. Nestor;P. Bayer;Guannan Wang;A. Ilyasova;Clément E Gille;Bryce E. H. Soraru;Kosala Ranathunge;A. Severn-Ellis;Ricarda Jost;W. Scheible;M. Dassanayake;Jacqueline Batley;D. Edwards;Hans Lambers;P. Finnegan
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作者：
Toby Bird;B. J. Nestor;P. Bayer;Guannan Wang;A. Ilyasova;Clément E Gille;Bryce E. H. Soraru;Kosala Ranathunge;A. Severn-Ellis;Ricarda Jost;W. Scheible;M. Dassanayake;Jacqueline Batley;D. Edwards;Hans Lambers;P. Finnegan
研究背景和目的平卧哈克（Hakeaprostrata）是一种磷高效利用植物，原产于澳大利亚西南部。它在低叶磷（P）下保持高光合速率，并表现出延迟的叶片绿化，这是一种提高养分利用效率的趋同适应。这项研究的目的是提供广泛的生理和基因表达谱叶发育，揭示途径导致从嫩叶作为营养库成熟叶作为低营养，energy-transmittingsources.MethodsTo探讨延迟绿化的基因表达，我们分析了从头转录组为H。叶发育的五个阶段。光合作用和呼吸速率，和叶片色素，P和氮（N）浓度进行了测定，包括P分为5个生化fracture.Key resultsTranscripts编码功能与叶结构的丰富度一般下降整个叶发育，伴随着叶片浓度的下降85%的花青素，90%的P和70%的N。与光合功能相关的基因的表达在叶片扩展期间或之后增加，与光合色素和活性的增加平行，在叶片发育中比没有延迟绿化的物种晚得多。随着叶的发育，胞质和线粒体核糖体蛋白的转录丰度普遍下降，而叶绿体核糖体蛋白increased.ConclusionsThere是一个更长的时间分离的叶细胞生长的叶绿体发育inH。在缺乏延迟绿化的物种中发现了prostratathan。转录组引导的H. prostrata提供了关于延迟绿化作为严重缺磷景观中的一种养分节约战略的见解。
Background and aimsHakea prostrata(Proteaceae) is a highly phosphorus-use-efficient plant native to southwest Australia. It maintains a high photosynthetic rate at low leaf phosphorus (P) and exhibits delayed leaf greening, a convergent adaptation that increases nutrient-use efficiency. This study aimed to provide broad physiological and gene expression profiles across leaf development, uncovering pathways leading from young leaves as nutrient sinks to mature leaves as low-nutrient, energy-transducing sources.MethodsTo explore gene expression underlying delayed greening, we analysed a de novo transcriptome forH. prostrataacross five stages of leaf development. Photosynthesis and respiration rates, and foliar pigment, P and nitrogen (N) concentrations were determined, including the division of P into five biochemical fractions.Key resultsTranscripts encoding functions associated with leaf structure generally decreased in abundance across leaf development, concomitant with decreases in foliar concentrations of 85% for anthocyanins, 90% for P and 70% for N. The expression of genes associated with photosynthetic function increased during or after leaf expansion, in parallel with increases in photosynthetic pigments and activity, much later in leaf development than in species that do not have delayed greening. As leaves developed, transcript abundance for cytosolic and mitochondrial ribosomal proteins generally declined, whilst transcripts for chloroplast ribosomal proteins increased.ConclusionsThere was a much longer temporal separation of leaf cell growth from chloroplast development inH. prostratathan is found in species that lack delayed greening. Transcriptome-guided analysis of leaf development inH. prostrataprovided insight into delayed greening as a nutrient-saving strategy in severely phosphorus-impoverished landscapes.