In vitro Antitumor Activities of Platycarya strobilacea Sieb et Zucc Infructescence Extracts
In vitro Antitumor Activities of Platycarya strobilacea Sieb et Zucc Infructescence Extracts
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DOI：
10.4314/tjpr.v13i6.4
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发表时间：
2014-09
影响因子：
0.6
通讯作者：
Liangliang Zhang;Yongmei Wang;Man Xu
中科院分区：
文献类型：
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作者：
Liangliang Zhang;Yongmei Wang;Man Xu
目的：评价松果提取物对A549、HepG2、SH-SY-5Y、HCT116和U2OS-NKFB细胞株的抗肿瘤活性。方法：采用Folin-Ciocalteu试剂法，测定了三种溶剂提取物(甲醇、乙酸乙酯和水)中总酚类物质的含量。用3-(4，5-二甲基噻唑-2-基)-2，5-二苯基四氮唑溴化法测定了不同溶剂提取物和单宁对5种癌细胞株(A549、HepG2、SH-SY-5Y、HCT116和U2OS-NKFB)的细胞毒作用。结果：乙酸乙酯提取物的酚类含量最高(725.02 mg GAE/g)，其次是甲醇提取物(627.64 mg GAE/g)。其中，乙酸乙酯提取物对A549、HEPG2、HCT116和U2OS-NKFB细胞有较强的抗癌活性，IC50值分别为40.1、42.6、40.1和73.4μg/mL。甲醇提取物对SH-SY-5Y细胞的细胞毒作用最强，IC50值为31.2μg/mL。随着乙酸乙酯和甲醇提取物浓度的增加，存活的癌细胞数量减少。结论：本研究证实了球果紫云英乙酸乙酯和甲醇提取物的抗肿瘤活性。因此，植物提取物是一种潜在的治疗材料，需要进一步的研究和开发。关键词：悬铃木，抗肿瘤，酚类化合物，单宁，四甲基偶氮唑盐比色法
Purpose: To evaluate the antitumor activities of Platycarya strobilacea infructescence extracts in A549, HepG2, SH-SY-5Y, HCT116, and U2OS-NKFB cell lines. Methods : The total amount of phenolics in P. strobilacea infructescence based on three solvent extracts (methanol, ethyl acetate and water) was measured using Folin-Ciocalteu reagent method. The cytotoxic effect of the various solvent extracts and tannins on five cancerous cell lines (A549, HepG2, SH-SY-5Y, HCT116, and U2OS-NKFB) were assayed using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide [MTT]. Results: Ethyl acetate extract had the highest phenolic content (725.02 mg GAE/g), followed by methanol extract (627.64 mg GAE/g). Among the extracts tested, ethyl acetate extract had effective anticancer activity against A549, HepG2, HCT116 and U2OS-NKFB cells with IC 50 values of 40.1, 42.6, 40.1 and 73.4 μg/mL, respectively. Methanol extract had the strongest cytotoxic effect on SH-SY-5Y cell with IC 50 value of 31.2 μg/mL. The number of surviving cancerous cells decreased as the concentration of the ethyl acetate and methanol extracts increased. Conclusion : The study confirms the antitumor activities of ethyl acetate and methanol extracts of P. strobilacea infructescence. Thus, the plant extracts constitute potential therapeutic materials that require further studies and development. Keywords: Platycarya strobilacea , Antitumor, Phenolic compounds, Tannins, MTT assay