Assessment of radical scavenging capacity and lipid peroxidation inhibiting capacity of antioxidant
Assessment of radical scavenging capacity and lipid peroxidation inhibiting capacity of antioxidant
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DOI：
10.1021/jf800605x
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发表时间：
2008-09-24
影响因子：
6.1
通讯作者：
Yoshida, Yasukazu
中科院分区：
文献类型：
--
作者：
Niki, Etsuo;Omata, Yo;Yoshida, Yasukazu
自由基清除抗氧化剂对抗氧化应激的作用受到广泛关注，并通过多种方法评估其抗氧化能力。其中，测量抗氧化剂对探针衰变影响的方法是应用最广泛的方法之一。本研究的目的是比较两种评估抗氧化能力的方法，一种是跟踪探针的衰变，另一种是测量人血浆中的脂质过氧化产物。结果表明，探针衰变法适用于评估抗氧化剂的自由基清除能力，但不适合评估其抑制血浆脂质过氧化的能力。无论使用亲水性探针还是亲脂性探针都是如此。这种不同的结果是由于抗氧化剂抑制脂质过氧化的功效取决于抗氧化剂衍生自由基的命运和抗氧化剂之间的相互作用以及自由基清除能力。因此，抗氧化剂抑制脂质过氧化的能力应该根据对氧化程度的影响来评估，而不是根据对探针衰变的影响来评估。
The role of radical scavenging antioxidants against oxidative stress has received much attention, and the antioxidant capacity has been assessed by various methods. Among them, a method that measures the effect of antioxidant on decay of the probe is one of the most widely used methods. The present study was performed to compare the two methods to assess the antioxidant capacity, one to follow the decay of the probe and the other to measure lipid peroxidation products in human plasma. It was shown that the method following probe decay was suitable for assessment of radical scavenging capacity of antioxidant, but not for the capacity to inhibit lipid peroxidation in plasma. This is true whether a hydrophilic or lipophilic probe is used. Such different results arise from the fact that the efficacy of inhibition of lipid peroxidation by antioxidants depends on the fate of antioxidant-derived radical and interaction between antioxidants as well as the capacity of free radical scavenging. Thus, the capacity of antioxidants for inhibition of lipid peroxidation should be assessed from the effect on the extent of oxidation, not from the effect on probe decay.