Normal development, wound healing, and adenovirus susceptibility in β5-deficient mice
Normal development, wound healing, and adenovirus susceptibility in β5-deficient mice
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DOI：
10.1128/mcb.20.3.755-759.2000
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发表时间：
2000-02-01
影响因子：
5.3
通讯作者：
Sheppard, D
中科院分区：
文献类型：
--
作者：
Huang, XZ;Griffiths, M;Sheppard, D
整合素已被证明在胚胎发育，伤口愈合，转移和其他生物学过程中起着重要作用。 Alpha V Beta 5是一种含RGD的细胞外基质蛋白的受体，已建议在皮肤伤口愈合和腺病毒感染中很重要。为了检查该受体的体内功能，我们使用胚胎干细胞中的同源重组产生了缺乏β5表达的小鼠。纯合子的小鼠正常发展，生长和繁殖。从β5（ - / - ）小鼠收获的角质形成细胞表现出受损的迁移和粘附到Alpha Vβ5配体玻璃体蛋白的粘附。但是，在β5（ - / - ）和β5（+/+）小鼠中，皮肤伤口的愈合率没有差异。此外，从无效小鼠获得的角质形成细胞和气道上皮细胞显示，腺病毒感染效率等于野生型小鼠。这些数据表明，Alpha V Beta 5对于正常发育，繁殖，腺病毒感染或皮肤伤口的愈合不是必不可少的。
Integrins have been shown to play important roles in embryonic development, wound healing, metastasis, and other biological processes. alpha v beta 5 is a receptor for RGD-containing extracellular matrix proteins that has been suggested to be important in cutaneous wound healing and adenovirus infection. To examine the in vivo function of this receptor, we have generated mice lacking beta 5 expression, using homologous recombination in embryonic stem cells. Mice homozygous for a null mutation of the beta 5 subunit gene develop, grow, and reproduce normally. Keratinocytes harvested from beta 5(-/-) mice demonstrate impaired migration on and adhesion to the alpha v beta 5 ligand, vitronectin. However, the rate of healing of cutaneous wounds is not different in beta 5(-/-) and beta 5(+/+) mice. Furthermore, keratinocytes and airway epithelial cells obtained from null mice show adenovirus infection efficiency equal to that from wild-type mice. These data suggest that alpha v beta 5 is not essential for normal development, reproduction, adenovirus infection, or the healing of cutaneous wounds.