In Vivo Measurement of Age-Related Stiffening in the Crystalline Lens by Brillouin Optical Microscopy
In Vivo Measurement of Age-Related Stiffening in the Crystalline Lens by Brillouin Optical Microscopy
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DOI：
10.1016/j.bpj.2011.08.008
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发表时间：
2011-09-21
影响因子：
3.4
通讯作者：
Yun, Seok Hyun
中科院分区：
文献类型：
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作者：
Scarcelli, Giuliano;Kim, Pilhan;Yun, Seok Hyun
晶状体的生物物理和生物力学特性（例如粘弹性）长期以来一直与调节和视力问题有关，例如老花眼和白内障。然而，很难以非侵入方式测量这些参数。在这里，我们使用体内布里渊光学显微镜来表征材料在 GHz 频率下的声学特性并测量透镜的纵向弹性模量。我们获得了活体小鼠晶状体的三维弹性图，该图显示了晶状体皮层和核的生物力学异质性，具有高空间分辨率。对小鼠进行为期 2 个月的体内纵向研究显示，晶状体核出现了与年龄相关的明显硬化。我们发现布里渊弹性模量与传统机械技术在低频（类似于 1 Hz）下测得的杨氏模量之间存在非常好的相关性（对数线性）。我们的结果表明布里渊显微镜对于基础和动物研究以及临床眼科具有潜在的用途。
The biophysical and biomechanical properties of the crystalline lens (e.g., viscoelasticity) have long been implicated in accommodation and vision problems, such as presbyopia and cataracts. However, it has been difficult to measure such parameters noninvasively. Here, we used in vivo Brillouin optical microscopy to characterize material acoustic properties at GHz frequency and measure the longitudinal elastic moduli of lenses. We obtained three-dimensional elasticity maps of the lenses in live mice, which showed biomechanical heterogeneity in the cortex and nucleus of the lens with high spatial resolution. An in vivo longitudinal study of mice over a period of 2 months revealed a marked age-related stiffening of the lens nucleus. We found remarkably good correlation (log-log linear) between the Brillouin elastic modulus and the Young's modulus measured by conventional mechanical techniques at low frequencies (similar to 1 Hz). Our results suggest that Brillouin microscopy is potentially useful for basic and animal research and clinical ophthalmology.