Time delays between patient and laboratory selectively affect accuracy of helminth diagnosis
Time delays between patient and laboratory selectively affect accuracy of helminth diagnosis
复制标题
DOI：
10.1016/j.trstmh.2006.04.008
复制
发表时间：
2007-02-01
影响因子：
2.2
通讯作者：
Fine, P. E. M.
中科院分区：
文献类型：
--
作者：
Dacombe, R. J.;Crampin, A. C.;Fine, P. E. M.
肠道蠕虫感染的研究受到样本采集限制的影响，因为粪便中蠕虫卵的鉴定受到从宿主体内排出的时间的影响。在不同的研究条件下，可能需要不同的方法来优化诊断灵敏度。在马拉维农村的研究中，我们收集了从受试者生产到现场工作人员收集样本到实验室检查的不同时间延迟的粪便样本。粪便通过Kato-Katz（KK）或甲醛-乙醚浓缩（FEC）方法处理。钩虫和曼氏血吸虫是最常见的蠕虫。KK组钩虫患病率（270/988，27%）高于FEC组（191/988，19%）。根据处理步骤的时间对两种方法的结果进行比较。处理延迟不影响S的检索。mansoni当保存/冷藏延迟超过3 h时，观察到两种方法检测钩虫的灵敏度降低近50%。从冷藏或保存到实验室处理延迟1天也使两种方法对钩虫的敏感性降低了50%。由于钩虫卵在运输过程中的选择性减少，在研究多种蠕虫感染时必须小心。当样品没有保存时，这一点尤其重要，即使是在短时间内，甚至用福尔马林保存。(C)2006年皇家热带医学和卫生学会。由爱思唯尔有限公司出版。保留所有权利。
Studies of intestinal helminth infections are influenced by the constraints of sample collection, as identification of helminth ova in stools is affected by the time since evacuation from the host. Different methods may be required to optimise diagnostic sensitivity underdifferent study conditions. In the context of studies in rural Malawi, we collected stool samples with different time delays from production by subjects to sample collection by field staff, to examination in the laboratory. Stools were processed by Kato-Katz (KK) or formol-ether concentration (FEC) methods. Hookworm and Schistosoma mansoni were the most common helminths identified. The prevalence of hookworm was higher with KK (270/988, 27%) than with FEC (191/988, 19%). Comparison was made between the results from the two methods according to the timing of the processing steps. Delays in processing did not affect retrieval of S. mansoni. A decrease in sensitivity of almost 50% for detection of hookworm was observed with either method when preservation/refrigeration was delayed by more than 3 h. A delay of 1 day from refrigeration or preservation to laboratory processing also reduced the sensitivity for hookworm by 50% for both methods. Care must be taken in studies of multiple helminth infections owing to the selective reduction of hookworm ova during transport. This is particularly critical when samples are not preserved, even over short periods of time, and even with formalin preservation. (C) 2006 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.