Reclamation of Chinese herb residues using probiotics and evaluation of their beneficial effect on pathogen infection
Reclamation of Chinese herb residues using probiotics and evaluation of their beneficial effect on pathogen infection
复制标题
益生菌回收中药残渣及其对病原菌感染的有益作用评价
DOI：
10.1016/j.jiph.2016.11.013
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发表时间：
2017-11-01
影响因子：
6.7
通讯作者：
Shen, Liang
中科院分区：
文献类型：
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作者：
Meng, Fanjing;Yang, Shaoguo;Shen, Liang
中药渣对环境的污染和中药渣中药物成分的巨大浪费阻碍了中药企业的发展。为解决这一问题，对几种益生菌进行了试验，最终筛选出植物乳杆菌（Lactobacillus plantarum，HM 218749）用于健胃消食片药渣的再利用。建立小鼠幽门螺杆菌感染模型，以评价抗H。采用尿素酶活性试验、组织学成像、酶联免疫吸附试验（ELISA）和变性凝胶梯度电泳（DGGE）等方法对药渣发酵上清液的pylon侵染活性进行了研究。结果表明，中药渣发酵上清液能有效抑制尿素酶活性，减缓胃区细胞浸润，显著降低IL-6、IL-8和TNF-α的产生（P < 0.01）。
Environmental pollution caused by herb residues and the huge waste of medicinal ingredients contained in herb residues hinder the development of traditional Chinese medicine enterprises. To solve this problem, several probiotics were tested, and Lactobacillus plantarum (HM218749) was finally selected for the reuse of herb residues of Jianweixiaoshi tablets. A mouse model of Helicobacter pylon infection was developed to evaluate the anti-H. pylon infection activity of the herb residue fermentation supernatant using a urease activity test, histological imaging, an enzyme-linked immunosorbent assay (ELISA) and denaturing gel gradient electrophoresis (DGGE). The results demonstrated that the herb residue fermentation supernatant successfully inhibited urease activity, slowed cell infiltration in the gastric area and significantly reduced the production of interleukin-6 (IL-6), IL-8 and TNF-alpha in the treatment group (p