Hypertonic saline resuscitation reduces neutrophil margination by suppressing neutrophil L selectin expression
Hypertonic saline resuscitation reduces neutrophil margination by suppressing neutrophil L selectin expression
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DOI：
10.1097/00005373-199807000-00002
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发表时间：
1998-07-01
影响因子：
--
通讯作者：
Junger, WG
中科院分区：
文献类型：
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作者：
Angle, N;Hoyt, DB;Junger, WG
高渗盐水（MS）通过抑制中性粒细胞氧化爆发和减少肺中性粒细胞内流来减轻创伤诱导的肺损伤。本研究调查了这是否是由HS对内皮粘附分子表达的影响、肺中化学引诱物的产生或HS对中性粒细胞选择素表达的直接影响引起的。BALB/c小鼠仅出血至40 mm Mg持续1小时，并用流出的血液和4 mL/kg 7.5% HS或两倍于流出血液体积的乳酸林格氏溶液（LRS），流式细胞术检测中性粒细胞L选择素表达，差异染色法检测中性粒细胞总数，免疫组化法检测肺内皮细胞P和E选择素表达，改良Boyden小室迁移法检测肺灌洗液中趋化因子。MS处理的动物的灌洗液的趋化活性与LRS处理的动物没有显著差异，HS复苏并没有改变内皮细胞P和E选择素的表达，然而，MS处理的动物的中性粒细胞表达的L选择素比LRS处理的小鼠的少。与MS处理的小鼠相比，LRS处理的动物的循环中性粒细胞计数显著降低。HS对肺中内皮选择素表达和趋化因子产生的影响很小，HS显著降低中性粒细胞L选择素表达，然而，这表明MS复苏可能通过阻止中性粒细胞L选择素表达和内皮细胞粘附来减轻肺损伤。
Hypertonic saline (MS) reduces hemorrhage-induced lung injury by suppressing the neutrophil oxidative burst and reducing lung neutrophil influx. This study investigated whether this is caused by the effects of HS on endothelial adhesion molecule expression, the production of chemoattractants in the lung, or a direct effect of HS on neutrophil selectin expression.Methods: BALB/c mice mere made to hemorrhage to 40 mm Mg for 1 hour and resuscitated with shed blood and either 4 mL/kg 7.5% HS or two times the shed blood volume of lactated Ringer's solution (LRS), Neutrophil L selectin expression was determined by flow cytometry, total neutrophil counts were obtained by differential staining, and pulmonary endothelial P and E selectin expression was evaluated by immunohistochemistry, Chemoattractants in lung lavages were determined with a modified Boyden chamber migration assa.Results: Chemotactic activity of lavage fluid of MS-treated animals was not significantly different from that of LRS-treated animals, and endothelial P and E selectin expression was not altered by HS resuscitation, Neutrophils of MS-treated animals, however, expressed significantly less L selectin than those of LRS-treated mice, Concomitantly, circulating neutrophil counts of LRS-treated animals were significantly decreased compared with those of MS-treated mice.Conclusion: HS had little effect on endothelial selectin expression and chemoattractant production in the lung, HS significantly decreased neutrophil L selectin expression, however, This suggests that MS resuscitation may reduce lung injury by preventing neutrophil L selectin expression and endothelial adhesion.