COLLECTION, DESCRIPTION AND TRANSFER OF EMBRYOS FROM CATTLE 10-16 DAYS AFTER ESTRUS
COLLECTION, DESCRIPTION AND TRANSFER OF EMBRYOS FROM CATTLE 10-16 DAYS AFTER ESTRUS
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DOI：
10.1530/jrf.0.0590205
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
MITCHELL, D
中科院分区：
文献类型：
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作者：
BETTERIDGE, KJ;EAGLESOME, MD;MITCHELL, D
在226头供体牛中，用妊娠血清促性腺激素诱导超数排卵，76.5%的供体牛有3次或3次以上的排卵。发情后10-16天在手术或屠宰时冲洗回收的卵子和胚胎占排卵数量的49.3%。在回收的胚胎中，73.3%为胚胎，平均产量为4.0个胚胎/处理母牛或4.8个胚胎/冲洗母牛。在65名供体中确定了卵子和胚胎的位置。在输卵管中偶尔发现胚胎和未受精卵（占回收的6.1%）。所有日期的子宫内均发现空透明带。胚胎的长度或直径在几天内和供体内变化极大，但平均值表明第10天和第16天之间呈对数生长。84个同步（.+-. 1天）的受体通过手术移植获得单胚胎，51个受体获得双胚胎。在第16天之前接受者中获得妊娠，但在第17天没有，这表明胚胎必须存在以防止黄体溶解的阶段。第42天的总体妊娠率为50.4%，另有18.1%的受体表现出延长的发情周期。在35名被允许进入足月的受体中，12人流产，最常见的是在第42天至第63天之间。
Of 226 donor cattle treated with pregnant mares serum gonadotropin to induce superovulation, 76.5% responded with 3 or more ovulations. Flushing at surgery or slaughter 10-16 days after estrus recovered eggs and embryos that represented 49.3% of the number of ovulations. Of those recovered, 73.3% were embryos, an average yield of 4.0 embryos/treated cow or 4.8 embryos/flushed cow. The location of eggs and embryos was determined in 65 of the donors. Embryos and unfertilized eggs (6.1% of those recovered) were occasionally found in the oviducts. Empty zonae pellucidae were also found in the uterus on all days. The lengths, or diameters, of embryos were extremely variable within days and within donors, but mean values indicated logarithmic growth between Days 10 and 16. Eighty-four synchronous (.+-. 1 day) recipients received single embryos, and 51 recipients twin embryos, by surgical transfer. Pregnancies were obtained in recipients up to Day 16 but not on Day 17, indicating the stage by which an embryo must be present to prevent luteolysis. The overall pregnancy rate of Day 42 was 50.4% and a further 18.1% of the recipients exhibited extended estrous cycles. Of 35 recipients that were allowed to go to term, 12 lost their pregnancies, most often between Days 42 and 63.