Age-related disturbance of memory and CREB phosphorylation in CA1 area of hippocampus of rats
Age-related disturbance of memory and CREB phosphorylation in CA1 area of hippocampus of rats
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DOI：
10.1016/j.brainres.2005.06.045
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发表时间：
2005-08-23
期刊：
影响因子：
2.9
通讯作者：
Kanba, S
中科院分区：
文献类型：
--
作者：
Kudo, K;Wati, H;Kanba, S
在长期记忆形成的早期过程中，环磷酸腺苷反应元件结合蛋白（CREB），一种多个信号转导途径汇聚的转录因子，已经被牵连。我们研究了背景恐惧条件反射（CFC）表现的年龄差异是否与海马CREB激活的变化有关，海马CREB是长期记忆的重要神经结构。用特异性识别CREB磷酸化形式的抗体（pCREB）以化学方法测定年轻（15周龄）和老年（120周龄）雄性大鼠海马中CREB的活化。年轻的大鼠表现出更好的性能比老年大鼠的冷冻时间在CFC。磷酸化的CREB所揭示的比例的pCREB-免疫反应阳性细胞的CREB-免疫反应阳性细胞的数量增加，在CA 1区，但不是在其他海马区训练CFC。行为表现与CA 1区CREB磷酸化之间的密切关系表明，海马CREB参与了与年龄相关的学习和记忆衰退。(c)2005 Elsevier B. V.保留所有权利。
In the early process of long-term memory formation, cyclic AMP response element-binding protein (CREB), a transcription factor on which multiple signal transduction pathways converge, has been implicated. We examined whether the age difference in the performance of contextual fear conditioning (CFC) is associated with a change in activation of CREB in the hippocampus which is an important neural structure for long-term memory. The activation of CREB in the hippocampus in young (15 weeks old) and old (120 weeks old) male rats was determined immunohistochemically with an antibody that specifically recognizes the phosphorylated form of CREB (pCREB). Young rats exhibited better performance than old rats with respect to the freezing time in CFC. Phosphorylation of CREB as revealed by the ratio of the pCREB-immunoreactive cell number to the CREB-immunoreactive cell number was increased in the CA1 region, but not in other hippocampal regions following training for CFC. The close relationship between behavioral performance and CREB phosphorylation in the CA1 region suggests that hippocampal CREB is involved in age-related decline of learning and memory. (c) 2005 Elsevier B.V. All rights reserved.