Retinoic acid affects craniofacial patterning by changing Fgf8 expression in the pharyngeal ectoderm
Retinoic acid affects craniofacial patterning by changing Fgf8 expression in the pharyngeal ectoderm
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DOI：
10.1111/j.1440-169x.2008.01069.x
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发表时间：
2008-12-01
影响因子：
2.5
通讯作者：
Wakisaka, Satoshi
中科院分区：
文献类型：
--
作者：
Abe, Makoto;Maeda, Takashi;Wakisaka, Satoshi
视黄酸信号在胚胎发育过程中建立正常模式和细胞分化中起重要作用。在这项研究中，我们表明，在胚胎第8.5天单次给予维甲酸会导致下颌到上颌样结构的同源异型转化。在第一咽弓近端区域Fgf8和Sprouty表达下调之前，还观察到间充质基因如Dlx5、Hand2、Tbx1和Pitx2的下调。维甲酸处理的胚胎口咽部严重收缩。与该观察结果一致，弓形内胚层和间充质中的Patched表达下调。因此，视黄酸影响上皮和间充质基因子集的表达，可能会破坏咽弓的区域特征。
Retinoic acid signaling plays important roles in establishing normal patterning and cellular differentiation during embryonic development. In this study, we show that single administration of retinoic acid at embryonic day 8.5 causes homeotic transformation of the lower jaw into upper jaw-like structures. This homeosis was preceded by downregulation of Fgf8 and Sprouty expression in the proximal domain of the first pharyngeal arch. Downregulation of mesenchymal genes such as Dlx5, Hand2, Tbx1 and Pitx2 was also observed. The oropharynx in retinoic acid-treated embryos was severely constricted. Consistent with this observation, Patched expression in the arch endoderm and mesenchyme was downregulated. Thus, retinoic acid affects the expression of subsets of epithelial and mesenchymal genes, possibly disrupting the regional identity of the pharyngeal arch.