Self-organized ZnO nanorod with photooxidative cell membrane perforation enables large-scale cell manipulation
Self-organized ZnO nanorod with photooxidative cell membrane perforation enables large-scale cell manipulation
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DOI：
10.1007/s00216-008-2226-2
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发表时间：
2008-06
影响因子：
4.3
通讯作者：
T. Saito;M. Seki;H. Tabata
中科院分区：
文献类型：
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作者：
T. Saito;M. Seki;H. Tabata
近年来，各种各样的设备被开发出来用于细胞功能的验证和应用。在我们之前的研究中，我们发现细胞膜的局部氧化反应可以在细胞中产生亚微米大小的可逆膜穿孔，而穿孔后80%以上的处理细胞仍然存活；因此，到目前为止，我们已经尝试了该机制的一些应用，并分析了它们的可行性。在目前的研究中，我们开发了一种杆状装置，其中利用光敏剂增加了膜穿孔的功能，并且使用该装置，我们已经尝试在大量细胞中产生膜穿孔。利用气-液-固机理合成了氧化锌纳米棒，并将α-三硫乙烯基（光敏剂）吸附在纳米棒顶部的金上，增加了穿膜功能。我们研究了在培养皿中，将棒体的生长侧与压在细胞上的基板接触，然后光敏剂光激发一定时间后，棒体对大鼠PC12细胞的氧化催化能力的影响。结果表明，在70 g/cm2的压力、0.82 W/cm2的光强、30 s的光照射下，添加在细胞外的水溶性荧光标记分子被细胞吸收，这与传统光化学细胞膜穿孔法针对单个细胞的情况相同。这些结果表明，细胞膜穿孔可以成功地实现在大量的细胞在同一时间。图自组织纳米棒的大规模细胞膜穿孔过程
Various devices have been developed for verification and application of cellular functions in recent years. In our previous study, we found that local oxidation reactions in the cell membrane could produce submicron sizes of reversible membrane perforations in cells, while more than 80% of treated cells were viable even after perforations; therefore, to date, we have attempted some applications of this mechanism and analyzed their feasibility. In the present study, we developed a rod-shaped device in which the function of membrane perforation is added by utilizing a photosensitizer and, using the device, we have attempted to produce membrane perforations in a large number of cells. Zinc oxide nanorods were synthesized on the basis of the vapor–liquid–solid mechanism and α-terthienyl (photosensitizer) was adsorbed onto gold at the top of the rods to add a membrane perforation function. We studied the effect of the oxidation catalytic ability of the rods on rat PC12 cells after pressing and making the rods’ growth side come into contact with the base plate pressed onto the cells in a culture plate followed by photoexcitation of the photosensitizer for a certain period of time. It was revealed that water-soluble fluorescent marker molecules added extracellularly were taken up by the cells when the rods were applied at a pressure of 70 g/cm2, with a light intensity of 0.82 W/cm2, and with light irradiation for 30 s, as found in the case of the conventional photochemical cell membrane perforation method targeted at a single cell. These results suggest that cell membrane perforation can be successfully achieved in a large number of cells at a time.FigureLarge-scale cell membrane perforation process using self-organized nanorods