Melanoma Inhibition by Anthocyanins Is Associated with the Reduction of Oxidative Stress Biomarkers and Changes in Mitochondrial Membrane Potential
Melanoma Inhibition by Anthocyanins Is Associated with the Reduction of Oxidative Stress Biomarkers and Changes in Mitochondrial Membrane Potential
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DOI：
10.1007/s11130-017-0638-x
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发表时间：
2017-12-01
影响因子：
4
通讯作者：
Jefferson, Andrew
中科院分区：
文献类型：
--
作者：
Diaconeasa, Zorita;Ayvaz, Huseyin;Jefferson, Andrew
花青素是水溶性色素，已被证明对健康有益。几项研究已经调查了它们对几种癌症的影响，但很少有人关注黑色素瘤。采用液相色谱-电喷雾质谱法（LC-ESI+-MS）测定了9种不同浆果样品的植物化学成分。经过之前的C-18 Sep-pak清理程序，鉴定出26种花青素。由于其化学模式不同，我们选择了富含蔓越莓和红葡萄花青素的提取物（C-ARE和RG-ARE）在正常和黑色素瘤细胞系上进行测试。C-ARE组合物由不同糖基化的花青素苷元组成；而RG-ARE则含有五种不同苷元的糖基化衍生物。C-ARE和RG-ARE花青素均可减少黑色素瘤细胞的增殖，增加氧化应激生物标志物，降低线粒体膜电位，对正常细胞无负面影响。协同反应可能归因于RG-ARE中存在的五种不同的苷元，事实证明，与花青素衍生物与不同糖（C-ARE）的混合物相比，RG-ARE对黑色素瘤细胞的作用更大。综上所述，C-ARE和RG-ARE花青素可能抑制黑色素瘤细胞增殖，增加氧化应激水平，而对正常细胞的作用相反。因此，花青素可作为化妆品和保健品行业的活性成分。
Anthocyanins are water soluble pigments which have been proved to exhibit health benefits. Several studies have investigated their effects on several types of cancer, but little attention has been given to melanoma. The phytochemical content of nine different berry samples was assessed by liquid chromatography followed by electrospray ionization mass spectrometry (LC-ESI+-MS). Twenty-six anthocyanins were identified, after a previous C-18 Sep-pak clean-up procedure. Chokeberry and red grape anthocyanins rich extracts (C-ARE and RG-ARE) were selected to be tested on normal and melanoma cell lines, due to their different chemical pattern. C-ARE composition consists of cyanidin aglycone glycosylated with different sugars; while RG-ARE contains glucosylated derivatives of five different aglycones. Both C-ARE and RG-ARE anthocyanins reduced proliferation, increased oxidative stress biomarkers and diminished mitochondrial membrane potential in melanoma cells, having no negative influence on normal cells. A synergistic response may be attributed to the five different aglycones present in RG-ARE, which proved to exert greater effects on melanoma cells than the mixture of cyanidin derivatives with different sugars (C-ARE). In conclusion, C-ARE and RG-ARE anthocyanins may inhibit melanoma cell proliferation and increase the level of oxidative stress, with opposite effect on normal cells. Therefore, anthocyanins might be recommended as active ingredients for cosmetic and nutraceutical industry.