Overexpression of ubiquitin-specific protease 2a (USP2a) and nuclear factor erythroid 2-related factor 2 (Nrf2) in human gliomas
Overexpression of ubiquitin-specific protease 2a (USP2a) and nuclear factor erythroid 2-related factor 2 (Nrf2) in human gliomas
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DOI：
10.1016/j.jns.2016.03.003
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发表时间：
2016-04-15
影响因子：
4.4
通讯作者：
Goudarzi, Peyman Karimi
中科院分区：
文献类型：
--
作者：
Boustani, Mohammad Reza;Khoshnood, Reza Jalili;Goudarzi, Peyman Karimi
背景：胶质瘤是最常见的成人原发性脑肿瘤之一。最近的研究表明，针对胶质瘤的分化和自我更新特征，有新的机会开发治疗方法。目的：本研究通过免疫组化染色检测胶质瘤患者中USP2a和Nrf2的表达水平及其与胶质瘤预后的关系。方法：本研究于2009年1月至2013年12月收集了40例原发性胶质瘤患者的组织样本。术前及术后24 h内分别行MRI检查。免疫组化检测USP2a和Nrf2的表达水平。数据分析采用SPSS 16.0、X-2检验、log-rank检验和Kaplan-Meier法。结果：神经胶质瘤细胞中USP2a的表达明显高于正常脑组织。USP2a染色升高与肿瘤分级（P = 0.02）和年龄（P = 0.016）显著相关。我们的研究结果表明，与正常脑组织相比，Nrf2在胶质瘤细胞中的表达明显更高。Nrf2高表达与年龄（P = 0.007）、肿瘤分级（P = 0.03）相关。Kaplan-Meier生存和log-rank分析显示，USP2a低表达患者的总生存期长于USP2a高表达患者（log-rank检验P < 0.001）。此外，Nrf2高表达患者的总生存期比低表达患者短（log-rank检验P < 0.001）。在单因素分析中，Nrf2和USP2a的高表达（P = 0.004; P = 0.006）、年龄（P = 0.025）和肿瘤分级（P = 0.001）与生存率低相关。多因素Cox比例风险模型显示，高Nrf2和USP2a染色（P = 0.001; P = 0.003）、晚期肿瘤分级（P = 0.01）和年龄（P = 0.033）是总生存期的独立预测因子。结论：综上所述，本研究结果提示USP2a和Nrf2可能是胶质瘤患者的预后指标。(C) 2016年Elsevier B.V.出版
Background: Gliomas are among the most frequent adult primary brain tumors. Recent studies have shown that there are novel opportunities for developing therapeutics by targeting the differentiation and self-renewal features of glioma.Objective: The aim of this study was to evaluate the expression levels of USP2a an Nrf2 in patients with glioma and their association with prognosis of gliomas that was detected with immunohistochemical staining.Methods: In this study, 40 patient's tissue samples with primary gliomas were collected between January 2009 and December 2013. MRI of patients was done before and within 24 h after surgery. USP2a and Nrf2 expression levels were examined by immunohistochemistry. Data were analyzed using the SPSS 16.0, X-2 test, log-rank test and Kaplan-Meier method.Results: lmmunohistochemistry indicated that USP2a expression was increased in glioma cells than normal brain tissues. The increased USP2a staining was markedly correlated with advanced tumor grade (P = 0.02) and age (P = 0.016). Our result showed that Nrf2 expression was significantly higher in glioma cells as compared to normal brain tissues. The high expression level of Nrf2 was markedly linked to age (P = 0.007), and tumor grade (P = 0.03). Kaplan-Meier survival and log-rank analysis indicated that patients with low expression of USP2a had longer overall survival than those with high levels (log-rank test P < 0.001). Moreover, patients with high Nrf2 expression had shorter overall survival than those with low levels (log-rank test P < 0.001). In the univariate analysis, the high expression of Nrf2 and USP2a (P = 0.004; P = 0.006), age (P = 0.025), and tumor grade (P = 0.001) were correlated with poor survival. Multivariate Cox proportional hazards model indicated that, high Nrf2 and USP2a staining (P = 0.001; P = 0.003), advanced tumor grade (P = 0.01) and age (P = 0.033) were independent predictor of overall survival.Conclusion: In summary, the result of this study showed USP2a and Nrf2 may be as prognostic marker in patients with gliomas. (C) 2016 Published by Elsevier B.V.