Sequence divergence in the MtDNA control region of amphidromous and landlocked forms of ayu
Sequence divergence in the MtDNA control region of amphidromous and landlocked forms of ayu
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DOI：
10.2331/fishsci.63.901
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发表时间：
1997-12-01
影响因子：
1.9
通讯作者：
Nishida, M
中科院分区：
文献类型：
--
作者：
Iguchi, K;Tanimura, Y;Nishida, M
为了探讨香鱼双游型和陆栖型的遗传差异，寻找区分这两种类型的遗传标记，对香鱼双游型和陆栖型的线粒体DNA差异进行了研究。对60条鱼的控制区的前半部分进行了测序，其中15条鱼来自2个两栖类和2个内陆种群。这些序列被发现是高度可变的，这表明它们的实用程序内的种内分歧的分析。内陆的形式是遗传上更均匀的多态性位点的数量和核苷酸多样性的大小方面比amendromous形式。型内净核苷酸替换在陆栖型中为0.024%，在两栖型中为-0.062%，表明型内遗传分化很小。相比之下，形式间的净核苷酸取代相当高，0.241- 0.527%，表明两种形式之间的线粒体基因组中的一些遗传异质性。近80%的鱼类可通过高变位点的核苷酸替换区分为无游或内陆。
We investigated mitochondrial DNA divergence for amphidromous and landlocked forms of ayu Plecoglossus altivelis to describe their genetic status and search for genetic markers discriminating the two forms. The first half of the control region was sequenced for 60 fish, 15 individuals from each of 2 amphidromous and 2 landlocked populations. These sequences were found to be highly variable, suggesting their utility for the analysis of intra-specific divergence. The landlocked form was genetically more homogeneous than the amphidromous form in terms of the number of polymorphic sites and the magnitude of nucleotide diversity. Intra-form net nucleotide substitutions were 0.024% in the landlocked and -0.062% in the amphidromous, showing that intra-form genetic differentiations were small. In contrast, inter-form net nucleotide substitutions were considerably higher, 0.241-0.527%, indicating some genetic heterogeneity in the mitochondrial genome between the two forms. Nearly 80% of fish were distinguishable as amphidromous or landlocked by the nucleotide substitutions at hyper-variable sites.